Full-automatic sample slide processing instrument and sample slide processing method

Abstract

The invention relates to the technical field of biology and particularly relates to a full-automatic sample slide treatment instrument and a sample slide treatment method. The invention provides a full-automatic sample slide treatment instrument. The full-automatic sample slide treatment instrument comprises a workbench, a slide frame for loading a sample slide, a control module and a mechanical arm electrically connected with the control module, wherein the workbench comprises a reaction area, a plurality of reaction tanks are arranged in the reaction area, and one reaction tank is used for containing one reaction liquid; the mechanical arm is arranged on the workbench, and the control module controls the mechanical arm to sequentially feed the slide rack into each reaction tank, so pre-hybridization pretreatment and / or post-hybridization elution of the sample slide are / is automatically completed. During use, reaction liquid is injected into each reaction tank, a sample slide is placed on the slide rack, the slide rack is fixed on the mechanical arm, and then the mechanical arm is controlled by the control module to sequentially feed the slide rack into each reaction tank, so pre-hybridization pretreatment and / or post-hybridization elution of the sample slide are / is automatically completed.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an automatic sample slide processing instrument and a sample slide processing method. Background technique [0002] Fluorescence in situ hybridization technology uses reporter molecules (such as fluorescent dyes, biotin, digoxin, etc.) to label nucleic acid probes, and then hybridizes the probes to the target DNA. If the two are homologous and complementary, the target DNA and nucleic acid can be formed. Probe hybrids. At this time, the reporter molecule can be used to perform qualitative, quantitative or relative positioning analysis on the target DNA under a fluorescence microscope. [0003] From the perspective of existing technologies, the main procedures of fluorescence in situ hybridization technology are: sample preparation, pretreatment, probe dripping, hybridization, post-hybridization elution and counterstaining, in which hybridization is incubated with a temperature-contr...

AI Technical Summary

Problems solved by technology

[0004] In the process of pretreatment or elution after hybridization, it is necessary to soak in at least two different test solution environments. The device has no automatic switching function, and the samples can only be switched to the test solution environment one by one manually. This process will bring artificial Individual errors in operation, specific errors include inconsistencies in the speed of manual pick-and-place samples, inconsistencies in the order of pick-and-place, and inconsistencies in the time between pick-and-place, etc. These individual errors in manual operations will affect the results of hybridization experiments to a certain extent, leading to The results of the hybridization experiment are inaccurate, and at the same time occupy a large amount of human resources. The reaction time of each process in the whole pretreatment and post-hybridization elution process is short, and it requires people to follow the operation throughout the process, which is a serious waste of labor.

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