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Encapsulated biochip

A biochip and chip support technology is applied in the field of low- and medium-density gene chips, protein chips, and encapsulated biochips. Achieve the effect of improving operation simplicity, ensuring quality and stability, and reducing costs

Active Publication Date: 2022-01-28
BEIJING BIONAXIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the low-to-medium density biochips currently on the market are often based on unpackaged bare gene chips, which generally need to be fenced and covered before hybridization. Cleaning after hybridization also requires special brackets and containers, resulting in biological The chip detection process is relatively complicated, and it cannot meet the requirements of convenient and fast POCT use, which limits the application of biochips in the field of POCT to a certain extent.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: The preparation, automatic liquid addition and detection process of packaged gene chip A are as follows:

[0035] 1) Preparation of low-medium density gene chips: design and synthesize probe 7 of oligonucleotides with amino-modified 5' end (entrusted to Shanghai Sangong Synthesis, Kunshan Propu and the company to synthesize), spotting instrument (imported Equipment: GESIM Nano-PlotterNP2.1 / E) dotted onto the substrate 6 through piezoelectric spraying (manufacturer: Boao Jingdian, type: glass substrate, specifications: length 75mm, width 25mm, thickness 1mm,), and the preparation is in progress The low-density gene chip is a gene chip with 10×10 array repeats (spot area: length 60 mm, width 18 mm).

[0036] 2) Preparation of the chip holder: the chip holder includes the bottom plate 1, the inner wall 2 and the outer wall 3 (customized by Liaoning Shenyang Shengang Plastic Co., Ltd. according to the above conditions), which are integrated by injection moldin...

Embodiment 2

[0046] Embodiment 2: The preparation, manual liquid addition and detection process of packaged gene chip B are as follows:

[0047] 1) Preparation of medium and low-density gene chips: design and synthesize probe 7 of oligonucleotides with amino-modified 5' end (synthesized by Shanghai Sangon), spotting instrument (imported equipment: GESIM Nano-Plotter NP2.1 / E) Through piezoelectric spraying onto the substrate 6 (manufacturer: Boao Jingdian, type: glass substrate, specifications: length 75mm, width 25mm, thickness 1mm), the prepared gene chip of medium and low density is 10×10 Gene chips with repeated arrays (spot area: length 60mm, width 18mm), the low-medium density gene chips, referred to as gene chips B.

[0048] 2) Preparation of the chip holder: the chip holder includes the bottom plate 1, the inner wall 2 and the outer wall 3 (customized by Liaoning Shenyang Shengang Plastic Co., Ltd. according to the above conditions), which are integrated by injection molding. The o...

Embodiment 3

[0054] Embodiment 3: The preparation, manual liquid addition and detection process of packaged gene chip C are as follows:

[0055] 1) Preparation of low-medium density gene chips: design and synthesize probe 7 of oligonucleotides with amino-modified 5' end (entrusted to Shanghai Sangong Synthesis, Kunshan Propu and the company to synthesize), spotting instrument (imported Equipment: GESIM Nano-PlotterNP2.1 / E) dotted onto the substrate 6 through piezoelectric spraying (manufacturer: Boao Jingdian, type: glass substrate, specifications: length 75mm, width 25mm, thickness 1mm,), and the preparation is in progress The low-density gene chip is a gene chip with 10×10 array repeats (spot area: length 60mm, width 18mm).

[0056] 2) Preparation of the chip holder: the chip holder includes the bottom plate 1, the inner wall 2 and the outer wall 3 (customized by Liaoning Shenyang Shengang Plastic Co., Ltd. according to the above conditions), which are integrated by injection molding. Th...

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Abstract

The embodiment of the invention provides a packaged biochip which comprises a biochip and a chip support, wherein the biochip comprises a substrate and a probe fixed on the substrate, and the probe is used for capturing target biomolecules in a sample to be detected and forming recognizable fluorescence signals after being excited; the chip support comprises a bottom plate, an inner side wall and an outer side wall; one surface, on which the probe is fixed, of the biochip is downwards arranged on the inner side wall of the chip support, so that a closed hybridization space defined by the biochip and the chip support is formed; and the bottom plate of the chip support is also provided with two symmetrical liquid holes and sealing plugs matched with the liquid holes. The packaged biochip is simple in structure and low in cost, can be directly used for hybridization, cleaning, fluorescence excitation, imaging detection and other processes of the chip, is convenient to use and operate, can also be applied to the field of POCT, and has remarkable economic significance and social benefits.

Description

technical field [0001] The present invention relates to the field of testing instruments and equipment, in particular to the technical field of in vitro diagnostic testing and molecular diagnostic testing, in particular to a packaged biochip, more specifically to a low-to-medium density gene chip for immediate detection and protein chip. Background technique [0002] Biochip (biochip), in the early literature, is consistent with the concept of gene chip. With the in-depth research of technology, biochip broadly refers to the integration of biochemical analysis process on the chip surface according to the principle of specific interaction between biomolecules. Thus realizing high-throughput rapid detection of DNA, RNA, peptides, proteins and other biological components. According to the different types of probes immobilized on the surface of the chip, biochips are divided into gene chips, protein chips, tissue chips, and cell chips. China's biochip research began in 1997-19...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C40B40/06C40B40/10C40B50/14C12Q1/6837G01N33/68G01N33/58
CPCC40B40/06C40B40/10C40B50/14C12Q1/6837G01N33/68G01N33/582C12Q2563/107C12Q2565/501
Inventor 华子昂刘宝全竹添朱美瑛万君兴张建李娜
Owner BEIJING BIONAXIN BIOTECH CO LTD
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