Near-infrared two-region activated probe and application thereof

A near-infrared and probe technology, applied in the field of nano biosensing, can solve the problems of indeterminacy and easy missed detection, and achieve the effect of accurate detection and high detection sensitivity

Pending Publication Date: 2022-02-01
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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Problems solved by technology

Existing identification methods include ultrasound, X-ray photography and biopsy, etc., all of which have certain limitations: first, in vivo detection methods are mainly morphological detection, which cannot be qualitative; second, biopsy combined with pathological analysis requires limited material Easy to miss

Method used

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  • Near-infrared two-region activated probe and application thereof
  • Near-infrared two-region activated probe and application thereof
  • Near-infrared two-region activated probe and application thereof

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Embodiment 1

[0069] This embodiment provides an activating probe for the detection of tumor sentinel lymph node metastasis of miRNA 155 highly expressed in breast cancer, including: a first-strand probe, a second-strand probe, a third-strand probe and a fourth-strand probe The needle, as well as the tumor-targeting peptide loaded on the delivery carrier, have targeting properties, imaging and detection functions simultaneously. In an embodiment, the above-mentioned fluorescent materials and nucleic acid sequences are all encapsulated in targeted functionalized modified stem cell vesicles to form an activated probe in the second near-infrared region.

[0070] 1. Preparation of near-infrared activated probes

[0071] (1) DNA chain design: using IDT-OligoAnalyzer TM Tool designs each nucleic acid sequence: fluorescent dye chain (F-DNA), quencher chain (Q-DNA), Linker-DNA, fuel chain (Fuel-DNA) and microRNA 155 (sequences are as SEQID No: 1-SEQ ID No: 5), all designed nucleic acid sequences ...

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Abstract

The invention discloses a near-infrared two-region activated probe and application thereof. The near-infrared two-region activated probe comprises a detection system and an amplification system, wherein the detection system is composed of a first chain probe, a second chain probe and a third chain probe, and the amplification system is composed of a to-be-detected target sequence, the second chain probe, the third chain probe and a fourth chain probe; the second-chain probe and the third-chain probe are completely complementary, and the first-chain probe and the fourth-chain probe are partially complementary with the third-chain probe in sequence; and a part of sequences in the third chain probe are completely complementary to the to-be-detected target sequence, and a part of sequences in the first chain and the fourth chain are partially identical to the to-be-detected target sequence. Toe-hold strand displacement reaction and near-infrared two-region fluorescence are combined, and the constructed near-infrared two-region activated probe can perform high-sensitivity, high-specificity and rapid detection on breast cancer sentinel lymph node metastasis on the living body level and has important significance on early diagnosis of breast cancer metastasis.

Description

technical field [0001] The invention relates to a molecular probe, in particular to a near-infrared two-region activated probe and its application in the detection of tumor sentinel lymph node metastasis, and belongs to the technical field of nanobiological sensing. Background technique [0002] In recent years, the morbidity and mortality of malignant tumors have been increasing. Late detection, easy metastasis, and difficult cure have always been challenging issues in cancer treatment. Breast cancer is the malignant tumor with the highest incidence rate among Chinese women, and its death rate also ranks fourth. Metastasis of breast cancer is the main cause of death of patients, and lymphatic metastasis is the main way of breast cancer spread. Positive identification of sentinel lymph nodes is the main means of defining lymphatic metastasis and also provides guidance for subsequent treatment. Existing identification methods include ultrasound, X-ray photography and biopsy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64C09K11/02
CPCG01N21/6428C09K11/025G01N2021/6432
Inventor 李春炎求瑞娟王强斌
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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