Application of Rv1860 protein, RV3881c protein, Rv2031c protein and Rv3803c protein in diagnosis of active tuberculosis infection

A technology of active pulmonary tuberculosis and rv3881c, applied in the field of disease diagnosis, can solve the problems of low positive rate, unable to meet clinical requirements, unable to solve the problem of negative bacteria detection, etc., and achieve the effect of high sensitivity

Pending Publication Date: 2022-03-08
江西省胸科医院
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Problems solved by technology

All methods have limitations. The sputum tuberculosis culture takes 2-8 weeks to obtain the culture results, and the positive rate is low, which cannot meet the clinical requirements. Although the sputum smear method has a short cycle, its specificity is poor; -MTB / RIF has fast detection speed and high sensitivity, but it cannot solve the detection problem of negative bacteria
Interferon-γ release test is also a method to assist in the diagnosis of tuberculosis, but it still cannot distinguish between ATB and LTBI

Method used

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  • Application of Rv1860 protein, RV3881c protein, Rv2031c protein and Rv3803c protein in diagnosis of active tuberculosis infection
  • Application of Rv1860 protein, RV3881c protein, Rv2031c protein and Rv3803c protein in diagnosis of active tuberculosis infection
  • Application of Rv1860 protein, RV3881c protein, Rv2031c protein and Rv3803c protein in diagnosis of active tuberculosis infection

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Embodiment 1

[0049] 1.1 Research data

[0050] 160 sera of patients with active tuberculosis (ATB), latent tuberculosis infection (LTBI) and healthy volunteers were collected. The age of the patients ranged from 16 to 93 years old. In the training phase, three groups of 180 serum samples (60 in each group) were used for microarray detection; in the verification phase, three groups of 300 serum samples (100 in each group) were further detected by ELISA. All samples were collected at Jiangxi Provincial Chest Hospital from December 2019 to September 2020, and the sample collection was approved by the Ethics Committee of Jiangxi Provincial Chest Hospital (No.: (2019) 50). Participants were registered after obtaining written consent and carried out in accordance with the Declaration of Helsinki (revised 2013).

[0051] ATB patients were diagnosed according to clinical symptoms, acid-fast bacilli (AFB) sputum smear, bacterial culture results and other criteria. The inclusion criteria for the ...

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Abstract

The invention relates to the field of disease diagnosis, in particular to application of an Rv1860 protein, an RV3881c protein, an Rv2031c protein and an Rv3803c protein in preparation of medicines for diagnosing active tuberculosis infection. The invention provides an application of a product for detecting the levels of an anti-Rv1860 antibody, an anti-RV3881c antibody, an anti-Rv2031c antibody and an anti-Rv3803c antibody in serum in preparation of a product for identification, diagnosis, auxiliary diagnosis, screening and / or auxiliary screening of active tuberculosis infected patients. Experiments prove that a biomarker combination containing four antigens is obtained, and active tuberculosis infected patients can be effectively screened.

Description

technical field [0001] The present invention relates to the field of disease diagnosis, in particular to the use of Rv1860 protein, RV3881c protein, Rv2031c protein and Rv3803c protein in preparing and diagnosing active pulmonary tuberculosis infection. Background technique [0002] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis (MTB) infection. It is one of the ten leading causes of death. According to the World Health Organization tuberculosis report, by 2019, about one-third of the global population will be infected with Mycobacterium tuberculosis, and 1.4 million people will die from tuberculosis. 1 / 4-1 / 3 of Mycobacterium tuberculosis infection patients do not have any clinical symptoms, which is also known as latent tuberculosis infection (LTBI). About 2 billion people currently suffer from LTBI, and about 10% of them will develop active TB during their lifetime. As the immune status of infected individuals changes, timely diagnosis...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/68C07K14/35
CPCG01N33/5695G01N33/6893C07K14/35G01N2333/35G01N2469/20
Inventor 李杰孔欣怡陈茂伟邹俊张春兰陈中书宗佩兰张齐龙张学钰袁小兰
Owner 江西省胸科医院
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