Application of phloretin in preparation of antidepressant drug

A kind of phloretin, antidepressant technology, applied in the field of medicine

Pending Publication Date: 2022-03-18
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the antidepressant effect of phloretin

Method used

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  • Application of phloretin in preparation of antidepressant drug
  • Application of phloretin in preparation of antidepressant drug
  • Application of phloretin in preparation of antidepressant drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Effects of phloretin on chronic stress-induced depression-like behavior in mice

[0012] 1. Experimental method

[0013] 1.1 Establishment of chronic stress depression mouse model

[0014] The mice were alternately stimulated in various ways, 1-2 stimulations per day, and the order of stimulation was different every week. After continuous stimulation for 6 weeks, the chronic unpredictable mild stress (CUMS) depression mouse model was established. Mice received unpredictable mild stimuli as follows:

[0015]

[0016] 1.2 Grouping and administration of animals

[0017] Before the start of the experiment, the mice were adaptively fed for 7 days, and then randomly divided into five groups: blank group (Control), chronic stress model group (CUMS), chronic stress + fluoxetine group [CUMS + Flu (20mg / kg )], chronic stress + phloretin low dose group [CUMS + Phi (40mg / kg)] and chronic stress + phloretin high dose group [CUMS + Phi (80mg / kg)]. Three weeks after CUMS modeli...

Embodiment 2

[0031] Effects of phloretin on the increase of HMGB1 expression and the activation of microglia induced by chronic stress

[0032] 1. Experimental method

[0033] 1.1 Take the whole brain

[0034] The mouse was anesthetized by intraperitoneal injection of 10% chloral hydrate. After the mouse was completely anesthetized, the limbs of the mouse were fixed with a needle, the chest cavity was opened, the heart was exposed, and the heart was perfused with PBS and 4% paraformaldehyde. Tissues were soaked in 4% paraformaldehyde for later use.

[0035] 1.2 Immunofluorescence staining

[0036] Replace the solution with the brain tissue, and use 10%, 20% and 30% sucrose solution for gradient dehydration. When the whole brain completely sinks to the bottom, it means that the dehydration is successful. The brain tissue was frozen into slices with a thickness of 20 μm using a cryostat. The brain slices were permeabilized with 0.3% Triton-100 solution for 20 minutes, then blocked with 1...

Embodiment 3

[0040] Effects of phloretin on the release of pro-inflammatory cytokines induced by chronic stress

[0041] 1. Experimental method

[0042] 1.1 Preparation of hippocampal tissue homogenate

[0043] After the ethology of the mice, they were sacrificed by cervical dislocation, and the hippocampus tissues were taken out in an ice bath and quickly frozen in liquid nitrogen. Mix hippocampus tissue with normal saline at a ratio of 1:9, and grind and homogenize with a homogenizer until no tissue fragments are visible. The hippocampal tissue homogenate was placed at 4°C for later use.

[0044] 1.2 Detection of inflammatory factors

[0045]ELISA kits were used to detect the contents of IL-1β, IL-6 and TNF-α in hippocampal tissue. The operation steps were carried out according to the instructions of the kit, and the OD value of each well was measured at the specified wavelength, and then the protein expression levels of IL-1β, IL-6 and TNF-α in the sample were calculated according t...

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Abstract

The invention discloses application of phloretin in preparation of antidepressant drugs, and belongs to the technical field of medicines. A mouse depression model is established through chronic unpredictable mild stimulation, the anti-depression effect of the phloretin is evaluated through behavioral detection means such as an open field experiment, a sweet water preference experiment, a tail suspension experiment and a forced swimming experiment, and it is proved through an immunofluorescence experiment and ELISA kit detection that the phloretin can inhibit microglial cell activation; the compound can relieve neuroinflammation and play a neuroprotective role, and can be used for preparing anti-depression drugs.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of phloretin in the preparation of antidepressant drugs. Background technique [0002] Depression is a global human mental disease, clinically characterized by significant and persistent low mood, with high prevalence and high recurrence rate, and the suicide rate of depressed patients continues to increase year by year. Depression is caused by a combination of genetic, biological, environmental and psychological factors, and treatment varies from person to person. The pathogenesis of depression is complex and has not been fully elucidated so far. Currently commonly used antidepressants mainly play a role by regulating certain chemical substances in the brain that control emotions or stress. Therefore, it is of great significance to study the pathogenesis of depression and to study effective therapeutic drugs for different pathogenesis. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/12A61P25/24
CPCA61K31/12A61P25/24
Inventor 徐丽星黄海琴赵咏梅
Owner NANTONG UNIVERSITY
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