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Poria cocos total polysaccharide as well as extraction method and application thereof

An extraction method and technology for total polysaccharide, applied in the field of total polysaccharide of Poria cocos and its extraction, can solve problems such as cumbersome production process, no public report, residual chemical reagents, etc., and achieve the effects of simple operation, low production cost and high reuse rate

Active Publication Date: 2022-04-08
HUBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, water-soluble polysaccharides and alkali-soluble polysaccharides are extracted separately when extracting Poria cocos polysaccharides, the yield is not high, and the loss is too much, or the alkali-soluble polysaccharides are extracted after derivatization, and the molecular weight and structure often change. , the production process is cumbersome and complicated, and there are still chemical reagent residues. At present, there is no public report on the method of combining Poria cocos alkali-soluble polysaccharides and water-soluble polysaccharides.

Method used

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  • Poria cocos total polysaccharide as well as extraction method and application thereof
  • Poria cocos total polysaccharide as well as extraction method and application thereof
  • Poria cocos total polysaccharide as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Select 5 g of dried Poria cocos sclerotia powder, add 80% ethanol to reflux extraction twice, extract for 2 hours and 1 hour respectively, and filter with a 150-mesh filter cloth to obtain filter residue 1; the amount of ethanol added each time is 30 mL.

[0069] After drying the filter residue 1, place it in 0.3mol / L KOH solution, the mass volume ratio of the filter residue 1 to KOH solution is 1g:20mL, 120r / min, stir for 3.0h, filter with a 150-mesh filter cloth, and take the supernatant To obtain the Poria base extract; add citric acid to the Poria base extract for neutralization, adjust the pH to 7, and filter with a 150-mesh filter cloth to obtain a gel-like solid.

[0070] The above-mentioned gel-like solid is washed 3 times with pure water, and the amount of water added each time is 3 times the amount of the gel-like solid, filtered through a 150-mesh filter cloth, the supernatant is combined to obtain a washing liquid, and the filter residue is collected to obtai...

Embodiment 2

[0074] Select 5 g of dried Poria cocos sclerotia powder, add 75% ethanol to reflux extraction twice, extract for 2 hours and 1 hour respectively, and filter with a 200-mesh filter cloth to obtain filter residue 1; the amount of ethanol added each time is 50 mL.

[0075] After drying the filter residue 1, place it in 0.4mol / L KOH solution, the mass volume ratio of the filter residue 1 and KOH solution is 1g:30mL, 130r / min, stir for 1h, filter through a 200-mesh filter cloth, and take the supernatant to obtain Poria cocosine extract; add phosphoric acid to the poria cocosine extract for neutralization, adjust the pH to 6.5, and filter with a 200-mesh filter cloth to obtain a gel-like solid.

[0076] The above-mentioned gel-like solid is washed 5 times with pure water, and the amount of water added each time is 4 times the amount of the gel-like solid, filtered through a 200-mesh filter cloth, the supernatant is combined to obtain a washing liquid, and the filter residue is collec...

Embodiment 3

[0080] Select 5 g of dried Poria cocos sclerotia powder, add 85% ethanol to reflux extraction for 3 times, extract for 2 hours, 1 hour and 1 hour respectively, and filter with a 250-mesh filter cloth to obtain filter residue 1; the amount of ethanol added each time is 40 mL.

[0081] After drying the filter residue 1, place it in 0.7mol / L KOH solution, the mass volume ratio of the filter residue 1 to the KOH solution is 1g:24mL, 100r / min, stir for 2h, filter through a 250-mesh filter cloth, take the supernatant to obtain Poria base extract; add hydrochloric acid to neutralize the Poria base extract, adjust the pH to 6.0, and filter with a 250-mesh filter cloth to obtain a gel-like solid.

[0082] The above-mentioned gel-like solid is washed 6 times with pure water, and the amount of water added each time is 5 times the amount of the gel-like solid, filtered through a 250-mesh filter cloth, the supernatant is combined to obtain a washing liquid, and the filter residue is collect...

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Abstract

The invention relates to poria cocos total polysaccharide and an extraction method and application thereof, and belongs to the technical field of traditional Chinese medicine extraction. The extraction method comprises the following steps: refluxing poria cocos sclerotium powder in ethanol for 1-3 times to obtain filter residues 1; mixing the filter residue 1 with a KOH solution, reacting for 0.5-3 hours to obtain an alkali extracting solution, and adjusting the pH value of the alkali extracting solution to 6-7 to obtain a gel solid; washing the gel-like solid for 3-6 times by using water, combining filtrates to obtain water washing liquid, and collecting filter residues to obtain the poria cocos alkali-soluble polysaccharide; performing microfiltration on the water washing liquid, collecting permeate and trapped fluid 1, performing ultrafiltration on the permeate, and collecting the trapped fluid to obtain trapped fluid 2; and combining the trapped fluid 1, the trapped fluid 2 and the poria cocos alkali-soluble polysaccharide, and drying to obtain the poria cocos total polysaccharide. The yield of the pachymaran obtained by the extraction process disclosed by the invention reaches 65.72 to 88.94 percent, and the content of the pachymaran reaches 76.20 to 96.14 percent.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to a total polysaccharide of Poria cocos and its extraction method and application. Background technique [0002] Poria cocos polysaccharides account for about 70-90% of the total weight of Poria cocos sclerotium, of which water-soluble polysaccharides account for about 3%, and the rest are water-insoluble alkali-soluble polysaccharides. Poria cocos alkali-soluble polysaccharides have not been utilized in the clinical use of traditional Chinese medicine because of their water insolubility. In the prior art, water-soluble polysaccharides and alkali-soluble polysaccharides are extracted separately when extracting Poria cocos polysaccharides, the yield is not high, and the loss is too much, or the alkali-soluble polysaccharides are extracted after derivatization, and the molecular weight and structure often change. , the production process is cumbersome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A23L33/125A61K31/715A61P25/24
Inventor 叶晓川韩思婕罗心遥刘丹魏琼
Owner HUBEI UNIV OF CHINESE MEDICINE
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