BHK21 suspension cell clone strain capable of enabling foot-and-mouth disease labeled virus to have strong growth adaptability and screening method of BHK21 suspension cell clone strain

A suspension cell and screening method technology, applied in the field of BHK21 suspension cell clones, can solve the problems of cumbersome culture steps and long culture cycle time, and achieve the effects of eliminating side reactions, reducing the number of inoculations, and improving immune efficacy.

Pending Publication Date: 2022-04-12
内蒙古必威安泰生物科技有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cell expansion culture steps are cumbersome and the culture cycle time is long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • BHK21 suspension cell clone strain capable of enabling foot-and-mouth disease labeled virus to have strong growth adaptability and screening method of BHK21 suspension cell clone strain
  • BHK21 suspension cell clone strain capable of enabling foot-and-mouth disease labeled virus to have strong growth adaptability and screening method of BHK21 suspension cell clone strain
  • BHK21 suspension cell clone strain capable of enabling foot-and-mouth disease labeled virus to have strong growth adaptability and screening method of BHK21 suspension cell clone strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0026] Hereinafter, specific embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0027] If not specified, the technical means used in the examples are conventional means of those skilled in the art; if not specified, the biological and chemical materials in the examples are commercially available.

[0028] The present invention provides a kind of screening method that makes the BHK21 suspension cell clone strain of foot-and-mouth disease marker virus growth adaptability strong, comprises the steps:

[0029] (1) Preparation of BHK21 suspension cell clones:

[0030] a. Recovery and culture of BHK21 suspension cells: 2 tubes of frozen BHK21 suspension cells (commercially purchased from the Culture Collection Center of the China Suspension medium of fetal bovine serum (BS-SMF IV serum-free medium, from Suzhou Womei Biotechnology Co., Ltd., the brand is advanced) was cultivated in a 100ml cell culture flask. When the cel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a screening method of a BHK21 suspension cell clone strain, and the BHK21 suspension cell clone strain obtained by the screening method, in particular to the BHK21-KLXF-QZ5 suspension cell clone strain. The invention also relates to an application of the BHK21 suspension cell clone strain in preparation of a virus antigen. The BHK21 suspension cell clone strain provided by the invention solves the problem of low virus antigen content.

Description

technical field [0001] The invention relates to a BHK21 suspension cell clone strain and a screening method thereof which can make the foot-and-mouth disease marker virus (especially the foot-and-mouth disease marker virus type O and type A bivalent 3B protein epitope deletion virus) grow adaptable. Background technique [0002] Foot-and-mouth disease (FMD) is a disease caused by foot-and-mouth disease virus (FMDV) infecting artiodactyls (such as pigs, cattle, sheep, deer, etc.), and is characterized by Infected cloven-hoofed animals will develop blisters on the mouth, feet and other parts of the skin, resulting in the death of some animals and greatly affecting the development of the animal husbandry industry, so it has attracted the attention of all countries. [0003] Using reverse genetics technology, the non-structural protein dominant epitope gene of the virus was deleted and transformed, and the foot-and-mouth disease type O and A type bivalently labeled vaccines were...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/071C12N5/02C12Q1/02C07K14/09A61K39/135A61P31/14
Inventor 徐婉英刘延麟张涛刘月梁玮白满达马红艳武俊兰李丹王国华刘冬冬刘洪明周灵芝王颖张七斤
Owner 内蒙古必威安泰生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap