BHK21 suspension cell clone strain capable of enabling foot-and-mouth disease labeled virus to have strong growth adaptability and screening method of BHK21 suspension cell clone strain

Abstract

The invention provides a screening method of a BHK21 suspension cell clone strain, and the BHK21 suspension cell clone strain obtained by the screening method, in particular to the BHK21-KLXF-QZ5 suspension cell clone strain. The invention also relates to an application of the BHK21 suspension cell clone strain in preparation of a virus antigen. The BHK21 suspension cell clone strain provided by the invention solves the problem of low virus antigen content.

Description

technical field [0001] The invention relates to a BHK21 suspension cell clone strain and a screening method thereof which can make the foot-and-mouth disease marker virus (especially the foot-and-mouth disease marker virus type O and type A bivalent 3B protein epitope deletion virus) grow adaptable. Background technique [0002] Foot-and-mouth disease (FMD) is a disease caused by foot-and-mouth disease virus (FMDV) infecting artiodactyls (such as pigs, cattle, sheep, deer, etc.), and is characterized by Infected cloven-hoofed animals will develop blisters on the mouth, feet and other parts of the skin, resulting in the death of some animals and greatly affecting the development of the animal husbandry industry, so it has attracted the attention of all countries. [0003] Using reverse genetics technology, the non-structural protein dominant epitope gene of the virus was deleted and transformed, and the foot-and-mouth disease type O and A type bivalently labeled vaccines were...

AI Technical Summary

Problems solved by technology

Cell expansion culture steps are cumbersome and the culture cycle time is long

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