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A kind of anaerobic river channel sediment degrading bacteria and its application

A technology of river sediment and strains, applied in the field of environmental microorganisms, can solve problems such as human health and ecological environment risks, and achieve the effects of reduction and harmlessness, convenient operation, and low cost

Active Publication Date: 2022-05-27
FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the organisms in the sediment can easily ingest the pollutants in the sediment. After entering the food chain, the pollutants will be enriched in the organisms, posing serious risks to human health and the ecological environment.

Method used

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  • A kind of anaerobic river channel sediment degrading bacteria and its application
  • A kind of anaerobic river channel sediment degrading bacteria and its application
  • A kind of anaerobic river channel sediment degrading bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation, screening and performance measurement of anaerobic river sediment degrading bacteria

[0031] The media and components used are as follows:

[0032] Liquid medium: glucose 5 g / L, tryptone 3 g / L, beef meal 2 g / L, NaCl 4 g / L, K 2 HPO 4 0.75 g / L, KH 2 PO 4 0.75 g / L, FeSO 4 ·7H 2 O 0.05 g / L, MgSO 4 ·7H 2 O 0.3 g / L, (NH 4 ) 2 SO 4 3 g / L, cysteine ​​0.25 g / L, natural pH.

[0033] Solid medium: glucose 5 g / L, tryptone 3 g / L, beef meal 2 g / L, NaCl 4 g / L, K 2 HPO 4 0.75 g / L, KH 2 PO 4 0.75 g / L, FeSO 4 ·7H 2 O 0.05 g / L, MgSO 4 ·7H 2 O 0.3 g / L, (NH 4 ) 2 SO 4 3 g / L, cysteine ​​0.25 g / L, 15 g / L agar powder, natural pH.

[0034] Channel sediment liquid culture medium: channel sediment (total solid TS content is 11%, VS content is 7.8% of TS, both are mass fractions), add water to adjust the TS content of the sediment to 5%, and the pH value is 7.0.

[0035] Channel sediment solid medium: channel sediment (TS content is 11%, VS content...

Embodiment 2

[0042] Example 2: Biological identification of anaerobic river sediment degrading strain YE6

[0043] The genomic DNA of strain YE6 was extracted and used as a template to amplify the 16S rDNA of the strain using a pair of universal primers (27F, 1492R). The upstream primer was 27F (5'-AGAGTTGATCCTGGCTCA-3') and the downstream primer was 1492R (5'-GGTTACCTTGTTACGACTT-3').

[0044] The PCR reaction system (20 μL) is as follows: template DNA 0.5 μL, PCR Taqmix 10 μL, upstream and downstream primers 0.6 μL each, plus ddH 2 O to the reaction system to 20 μL.

[0045] PCR program: pre-denaturation at 94 °C for 5 min, denaturation at 94 °C for 30 s, annealing at 55 °C for 30 s, extension at 72 °C for 1 min 30 s, the above cycle was repeated 30 times, extension at 72 °C for 10 min, and finally stored at 4 °C.

[0046] The PCR product was purified and sequenced by Shanghai Jie Li Biotechnology Co., Ltd., and the 16S rDNA sequence of the obtained strain was shown in SEQ ID NO.1. The...

Embodiment 3

[0048] Example 3: Preparation of liquid bacterial agent for anaerobic river sediment degrading strain YE6

[0049] The pure YE6 strain was inoculated into 10 mL of liquid medium, cultured anaerobic at 37 °C for 24 h, and then transferred to the liquid medium of the next-level expansion culture with an inoculum of 5% of the liquid medium volume. Under the same conditions, multi-stage anaerobic expansion culture can be carried out to obtain the liquid inoculum of the strain.

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Abstract

The invention discloses an anaerobic river channel sediment-degrading bacterial species-Bacillus hainei Bacillus haynesii YE6 and its application in anaerobic degradation of river sediments relate to the field of environmental microbial technology. Bacterial strain of the present invention Bacillus hainei Bacillus haynesii YE6 has been preserved in the China Center for Type Culture Collection, its preservation number is CCTCC NO:M 20211674, and the preservation date is December 24, 2021. Bacillus hainei Bacillus haynesii YE6 can effectively improve the degradation rate of organic matter in river sediment in an anaerobic environment, and can be used for the reduction and harmless treatment of river sediment, and has high application value.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to an anaerobic river sediment-degrading bacteria and its application. Background technique [0002] With the rapid economic development and rapid population expansion, a large number of pollutants are discharged into the river, exceeding the self-purification capacity of the river, causing serious deterioration of the river and the surrounding environment, and negatively affecting people's lives and urban development. Many toxic and harmful inorganic substances and persistent organic substances remain in the river sediment for a long time and accumulate in large quantities, making them complex in composition and difficult to degrade. When environmental factors change, organic pollutants such as nitrogen and phosphorus in the sediment will permeate into the groundwater or be released into the overlying water. In addition, the organisms in the sedime...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/04C12R1/07C02F101/30
CPCC12N1/20C02F11/04C02F2101/30C02F2103/007C02F2303/06
Inventor 邱建贺翁雯周国彪梁锡伟
Owner FOSHAN YUHUANG ECOLOGICAL ENVIRONMENT TECH
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