Design method of capture probe, capture probe and application of capture probe

Abstract

The invention relates to the technical field of biology, and discloses a design method of a capture probe, the capture probe and application of the capture probe. The design method of the capture probe comprises the following steps: collecting a sample of a moss plant group, preprocessing the sample, and performing transcriptome sequencing to obtain original sequencing data; performing filtering and transcriptome assembly on the original sequencing data to obtain a protein sequence and a nucleotide sequence of the sample; carrying out clustering analysis on the protein sequences to obtain orthologous single-copy genes, and comparing and extracting nucleotide sequences of the orthologous single-copy genes to obtain target single-copy genes; the method comprises the following steps: screening a target single copy gene to obtain a target gene sequence, designing a DNA short sequence according to the target gene sequence, obtaining a complementary RNA short sequence according to the DNA short sequence, and synthesizing a capture probe according to the RNA short sequence. The capture probe provided by the invention is high in efficiency, low in cost and remarkable in enrichment effect when being applied to genome sequencing of moss plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for designing a capture probe, the capture probe and applications thereof. Background technique [0002] Bryophytes include liverworts (~7,300 species), mosses (~13,000 species), and hornworts (~250 species), and the phylogenetic relationships within these three clades are still lacking in systematic research. At present, scholars' research on the phylogeny of liverworts mainly adopts the method of joint analysis of a few molecular markers, which has problems such as insufficient molecular data, incomplete sampling, and insufficient support rate of the obtained phylogenetic tree, especially some rapid radiation evolution. Taxa (such as Carnationes) and the position of some problematic taxa (such as Carnations). [0003] The development of high-throughput sequencing (high-throughput sequencing) technology has rapidly transformed molecular systematics from the initial use of ...

AI Technical Summary

Problems solved by technology

However, the method of transcriptome sequencing is limited by the availability of fresh material of the species, and genome sequencing is not conducive to the application of large-scale phylogenetic genomics due to the large amount of data and high efficiency and low cost.

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