Nanobody targeting novel coronavirus and its preparation method and application

A nano-antibody and coronavirus technology, applied in the biological field, can solve the problem of lack of neutralizing antibodies of the new crown mutant strain Omicron and its subtype BA.2, and achieve the effects of mass screening, improved screening efficiency, and low production cost
CN114478757BActive Publication Date: 2022-07-05SHENZHEN PEOPLES HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHENZHEN PEOPLES HOSPITAL
Publication Date
2022-07-05

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Abstract

The present invention discloses a nanobody targeting novel coronavirus and a preparation method and application thereof. The nanobody comprises nanobody NbN2, nanobody NbN3, nanobody NbN4, nanobody NbN5, nanobody NbN6 and nanobody NbN11. At least one of the provided nanobodies against the RBD of the new coronavirus wild strain, the new coronavirus variant Alpha (Alpha), the new coronavirus Beta (Beta), the new coronavirus Delta (Delta), the new coronavirus Omicron and its subtype BA.2 The domain has a high affinity, which can quickly and specifically neutralize different new coronaviruses and effectively inhibit its activity; at the same time, the provided nanobody has a longer CDR3 sequence, which is conducive to the flexible adjustment of its conformation by the nanobody, improving the The combination with the virus is beneficial to wide application.
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Description

technical field

[0001] The present invention relates to the field of biotechnology, in particular to a nanobody targeting novel coronavirus and a preparation method and application thereof. Background technique

[0002] The novel coronavirus pneumonia (Corona Virus Disease 2019, COVID-19) can be transmitted through droplet transmission, contact with respiratory secretions, aerosol transmission, etc., and the consequences are very serious. The acute respiratory syndrome coronavirus 2 (Severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) that causes COVID-19 mainly expresses four structural proteins, namely, the spike protein (spike, S) and the envelope protein (envelope protein). ), membrane proteins (membrane) and nucleocapsid proteins. The S protein includes the N-terminal S1 subunit and the C-terminal S2 subunit. The S1 subunit contains a receptor binding domain (RBD) of about 200 amino acids, which is responsible for the binding of the virus to the host receptor,...

Claims

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