Kit for diagnosing malnutrition condition through flora detection of colon cancer tumor patient
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology for malnourished and tumor patients, applied in microorganism-based methods, microbial assays/tests, resistance to vector-borne diseases, etc., can solve abnormal defecation, blood-colored stools, and colon cancer patients affecting patient prognosis and treatment resistance. Acceptability and other issues to achieve accurate detection results
Pending Publication Date: 2022-06-07
ZHEJIANG CANCER HOSPITAL
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0002] Colon cancer is a malignant tumor that originates in the epithelium of the colonic mucosa. Patients with colorectal cancer generally have the following manifestations: 1) Abnormal defecation: When abnormal lesions occur in the colon, the patient will experience abnormalities such as diarrhea and constipation. With blood; 2) Abdominal pain: During the course of colon cancer, the tumor will grow rapidly in the intestinal cavity, which will block the intestinal cavity, causing partial intestinal obstruction or complete intestinal obstruction, and metabolites cannot be excreted and accumulate In the intestinal tract, severe abdominal pain occurs; 3) Inexplicable nausea: The intestinal tract is stimulated by damage, causing nausea, abdominal distension, etc.; 4) Malnutrition: The growth of t
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0037] Study on the intestinal flora of colon cancer under different nutritional status;
[0038] Nutritional status assessment methods and standards: nutritional status is assessed through the PG-SGA assessment tool. PG-SGA includes patient self-assessment sections (including body weight, food intake, symptoms, activity and physical function) and medical staff assessment sections (including related to nutrition). related disease status, metabolic status, and physical examination); the results of the qualitative score are as follows; 0-1 is well-nourished (PG-SGA1), 2-3 is suspected malnutrition (PG-SGA2), and 4-8 is moderate Severe malnutrition (PG-SGA3), ≥ 9 is classified as severe malnutrition (PG-SGA4);
[0039] The diagnostic power of classification and prediction of different microbiota species was examined according to the ROC curve, and the results were as follows: figure 1 Shown in A and 1B; the obtained results show that the diagnostic accuracy of Atopobium.Vaginae,...
Embodiment 2
[0044] Use of the kit for the detection of microbiota in patients with colon cancer and diagnosing malnutrition:
[0045] 1. Extract DNA template
[0046] The feces of colon cancer patients were sampled and stored in DNAhelper protective solution, and the ratio of fecal samples to DNAhelper protective solution was 1:2; genomic DNA was extracted.
[0047] 2. PCR amplification reaction
[0048] Use the PCR reaction kit from the detection kit, which contains the following primer pairs:
[0049] 1) Atopobium.Vaginae DNA forward primer:
[0050] 5'-GGTGAAGCAGTGGAAACACT-3'
[0051] Atopobium.Vaginae DNA reverse primer:
[0052] 5'-ATTCGCTTCTGCTCGCGCA-3'
[0053] 2) Selenomonassputigena DNA forward primer:
[0054] 5'-AGAGTTTGATCCTGGCTCAG-3'
[0055] Selemonassputigena DNA Reverse Primer:
[0056] 5'-CTCAATATTCTCAAGCTCGGTT-3'
[0057] 3) Faecalibacterium prausnitzii DNA forward primer:
[0058] 5'-CCCGGCATCGGGTAGAG-3'
[0059] Faecalibacteriumprausnitzii DNA reverse primer...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a kit for detecting and diagnosing malnutrition conditions of colon cancer tumor patients by flora, which comprises DNA (deoxyribonucleic acid) primer sequences of strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains, DNA primer sequences of the strains and DNA primer sequences of the strains, the kit further comprises an excrement sample preservation reagent, a PCR reaction reagent, a PCR product purification reagent and a sequencing reaction reagent. The method comprises the following steps: carrying out DNA extraction and PCR amplification on feces of a patient suffering from the colon cancer, then carrying out sequencing by using DNA sequencing primers of Atobium.Vaginae, Selenomonas sputigena and Faecalibacterium prausnitzii to obtain DNA sequence abundance of three strains in a sample, and diagnosing the nutritional status of the patient suffering from the colon cancer according to the correlation between the abundance of the three strains and the nutritional evaluation condition. The kit diagnosis method provided by the invention can noninvasively and accurately diagnose and evaluate the nutrition status of the colon cancer.
Description
technical field [0001] The invention belongs to the technical field of bacterial flora detection in colon cancer patients, in particular to a kit for detecting and diagnosing malnutrition status of colon cancer tumor patients. Background technique [0002] Colon cancer is a malignant tumor originating from the colonic mucosal epithelium. Patients with colorectal cancer generally have the following symptoms: 1) Abnormal defecation: when the colon has abnormal lesions, the patient will experience abnormal conditions such as diarrhea and constipation. 2) Abdominal pain: During the course of colon cancer, the tumor will grow rapidly in the intestinal lumen, which will block the intestinal lumen, causing partial or complete intestinal obstruction, and metabolites cannot be excreted and accumulated. In the intestine, severe abdominal pain occurs; 3) Inexplicable nausea: the intestine is stimulated by damage, nausea, abdominal distention, etc.; 4) Malnutrition: tumor growth needs t...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more