Boletus melanin hair dye as well as preparation method and use method thereof
A technology of melanin and boletus, applied in hair care, pharmaceutical formulations, cosmetic preparations, etc., can solve the problems of small molecular substances easily passing through the skin barrier, allergies, and carcinogenicity, and achieve non-toxic and harmless prices, raw materials Effects of natural, simple ingredients
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[0047] The present invention also provides a method for preparing the bolete melanin hair dye, comprising the following steps:
[0048] (1) Agent A: mix softener, pH regulator, thickener and water;
[0049] (2) Agent B: mix bolete melanin, pH regulator, thickener, moisturizing agent and water;
[0050] (3) Agent C: Mix the mordant, pH adjuster, thickener and water.
[0051] The present invention further provides the bolete melanin hair dye, and the method for using the bolete melanin hair dye prepared by the preparation method of the bolete melanin hair dye, comprising the following steps:
[0052] (1) Apply 1 / 2 of the total mass of Agent A to the hair, seal it with plastic wrap, and dye the hair at 20-40°C for 10-40 minutes;
[0053] (2) Mix the remaining agent A and agent B, apply it on the hair obtained in step (1), seal it with plastic wrap, and dye the hair at 25 to 50°C for 20 to 50 minutes;
[0054] (3) Apply agent C on the hair obtained in step (2), seal it with pla...
Embodiment 1
[0061] A preparation method of bolete melanin:
[0062] Boletus mycelium culture: The boletus mycelium is cultivated by liquid fermentation. The medium used was PDA, the culture conditions were 5% inoculum, 28°C, 280rpm, and cultured for 20d. Afterwards, the mycelium liquid was collected, the liquid was rotary evaporated and freeze-dried together with the mycelium, and the porcini mushroom powder was obtained after drying. (Working temperature -85℃, vacuum degree 2×10 -3 mbar, freeze-dried 3d).
[0063] Preparation of boletus melanin extract: using the ultrasonic-assisted-alkali-solution acid precipitation method, weigh the boletus powder, add 1mol / L NaOH solution at a ratio of 1:30 to the liquid, mix well, and place it in an ultrasonic cell disruptor for 20min , in a water bath at 60°C for 40min. Centrifuge at 10000r / min for 10min, collect the supernatant, adjust the pH of the supernatant to 1.5, take a water bath at 80°C for 12h, cool naturally, centrifuge at 10000r / min ...
Embodiment 2
[0065] A preparation method of bolete melanin:
[0066] Boletus mycelium culture: The boletus mycelium is cultivated by liquid fermentation. The medium used was PDA, the culture conditions were 6% inoculum, 29° C., 290 rpm / min, and cultured for 22 days. Afterwards, the mycelium liquid was collected, the liquid was rotary evaporated and freeze-dried together with the mycelium, and the porcini mushroom powder was obtained after drying. (Working temperature -88℃, vacuum degree 1.8×10 -3 mbar, freeze-dried 2d).
[0067] Preparation of boletus melanin extract: using ultrasonic-assisted-alkaline dissolution and acid precipitation method, weigh the boletus powder, add 1mol / L NaOH solution at a ratio of 1:32, mix well, and place it in an ultrasonic cell disruptor for 25min , in a water bath at 65°C for 45min. Centrifuge at 11000r / min for 8min, collect the supernatant, adjust the pH of the supernatant to 2.0, take a water bath at 80°C for 14h, cool naturally, centrifuge at 11000r / m...
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