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Colored barley grain anthocyanin transport related gene HvGST and application thereof

An anthocyanin and barley technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., to achieve the effects of the selection of new blue-grain barley varieties, the promotion of molecular breeding, and the rapid, efficient and accurate identification

Active Publication Date: 2022-08-02
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still few studies on the biosynthetic pathway and other loci of blue-grained barley anthocyanins

Method used

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  • Colored barley grain anthocyanin transport related gene HvGST and application thereof
  • Colored barley grain anthocyanin transport related gene HvGST and application thereof
  • Colored barley grain anthocyanin transport related gene HvGST and application thereof

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Embodiment Construction

[0047] The present invention will be further described in detail below with reference to the accompanying drawings, so that those skilled in the art can refer to the description and implement accordingly.

[0048] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the reagents and materials can be obtained from commercial sources unless otherwise specified.

[0049] The invention discloses a gene glutathione transferase related to anthocyanin transport in colored barley grains GST gene, named HvGST ; Two significantly associated QTL loci were identified in 4HL and 7HL mainly by genome-wide association analysis, among which the 7HL chromosome HvGST The gene is the first reported gene related to grain anthocyanin transport in Triticum HvGST A 203 bp base deletion at the end of the coding region results in a frameshift mutation that blocks anthocyanin transfer, resulting in white barl...

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Abstract

The invention discloses a colored barley grain anthocyanin transport related gene which is a gene (a), (b) or (c) as follows: the base sequence of (a) is shown as SEQ ID NO: 1, the base sequence of (b) is shown as SEQ ID NO: 2, and the base sequence of (c) is shown as SEQ ID NO: 3; the function of transferring anthocyanin from cytoplasm to vacuoles is provided in barley plants. The invention also discloses application of the colored barley grain anthocyanin transport related gene in developing a grain color related diagnostic marker aiming at candidate gene key variation, barley assisted selective breeding or preparing a barley assisted selective breeding kit. The method can realize rapid, efficient and accurate identification of colored barley and white barley materials, and has a promotion effect on new variety breeding and molecular breeding of the colored barley.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to colored barley grain anthocyanin transport related genes HvGST and its applications. Background technique [0002] Anthocyanins are natural water-soluble pigments that widely exist in plants, belong to flavonoids, and are also one of the main coloring substances of plants. Anthocyanin has a variety of biological functions, has the effect of scavenging free radicals and anti-oxidation, can prevent the oxidative damage of biological macromolecules such as proteins and nucleic acids caused by reactive oxygen species, and has anti-oxidation, anti-cancer, hypoglycemic, hypolipidemic , Prevention of cardiovascular and cerebrovascular diseases, antibacterial and other effects. [0003] Tibetan naked barley, commonly known as highland barley, is widely planted in the Qinghai-Tibet Plateau of my country. Highland barley contains a variety of anthocyanins, and the accumulation of different an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/11C12Q1/6895C12Q1/6858
CPCC12N9/1088C12Q1/6895C12Q1/6858C12Y205/01018C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 王春超郭刚刚徐东东达瓦顿珠张京
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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