Application of maltol in preventing and treating neuroretrogressive diseases
A maltol, prevention and treatment technology, applied in the application field of maltol in the preparation of drugs for the prevention and treatment of neurodegenerative diseases, can solve the problems of undiscovered food health care and medicinal effects
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Embodiment 1
[0022] Example 1 The protective effect of maltol on SH-SY5Y cell morphology
[0023] SH-SY5Y cells were cultured in 1640 medium with a seeding density of 10 5 / ml, after adding 2mmol / l maltol for 2 hours, add H 2 o 2 , 37 ℃ cell incubator for 10 hours, observe the cell morphology under an inverted microscope.
[0024] The result is as figure 1 A-E, H 2 o 2 After treatment, cells shrunk and deformed, with fuzzy borders and vacuoles inside, and the morphological changes were reduced after maltol protection.
Embodiment 2
[0025] Example 2 The growth-promoting effect of maltol on SH-SY5Y cells
[0026] The growth-promoting effect of maltol on cells was determined by MTT assay. The chemical name of MTT is 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyltetrazolium bromide, and the trade name is thiazolium blue (MTT for short), which can be Succinate dehydrogenase is reduced to insoluble blue-purple crystals and deposited in the cells, but MTT cannot be reduced to blue-purple crystals in dead cells. DMSO can dissolve the blue crystals, so by measuring The difference in absorbance at different wavelengths can reflect the growth status of cells.
[0027] The result is as figure 2 As shown, through the MTT experiment, the results showed that after maltol protection, the cell survival rate increased significantly, indicating that maltol can promote the growth of nerve cells.
Embodiment 3
[0028] Example 3 Protective effect of maltol on DNA damage in SH-SY5Y cells
[0029] 1. The protective effect of maltol on the formation of small DNA fragments in SH-SY5Y cells
[0030] During the process of cell apoptosis, the genomic DNA in the nucleus can be degraded by the activated endogenous endonuclease into a small fragment of integral multiples of 180-200 bp, which is a characteristic change of cell apoptosis. Intracellular DNA is extracted, analyzed by agarose gel electrophoresis, and detection of the existence of such small DNA fragments is one of the important indicators for testing cell apoptosis.
[0031] Specific steps are as follows:
[0032] Take 106 cells and wash twice in cold PBS; lyse in 2ml lysate (50mM Tris-HCl PH8; 20mM EDTA, PH8 2% SDS); overnight at 37°C; place on ice for 10min; add 0.8ml saturated NaCl, centrifuge at 3000rpm After 1 hour, take the supernatant, and centrifuge once under the same conditions; add RNase to the supernatant (final concen...
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