Transgenic insect

An insect and gene technology, applied in the field of genetic manipulation of insects, can solve problems such as difficulty in injecting DNA

Inactive Publication Date: 2003-04-09
IMPLYX
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0015] Therefore, the establishment of a reliable technique for introducing foreign genes into the Anopheles genome faces two major problems: 1) the development of a DNA delivery vector capable of successful transposition in Anopheles mosquitoes; and 2) overcoming the limitations of injecting DNA into mosquito embryos. Establishment of new technologies with technical difficulties

Method used

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  • Transgenic insect
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Embodiment

[0047] In the following experiments, a plasmid vector called MinHyg (shown in figure 1 ) to complete the integration of the heterologous gene into the Anopheles genome. Such as figure 1 As indicated, the green fluorescent protein gene GFPS65T (GFP) (Heim et al., Nature, 1995; 373: 663-664) was chosen as a reporter gene to show the successful integration of the completed DNA.

[0048] The actin promoter from the D. melanogaster actin5C gene was selected to drive expression of the GFPS65T marker (Fyrberg et al., Cell, 1983; 33:115-123).

[0049] A hygromycin gene under the control of the inducible heat shock protein 70 (hsp70) promoter was also incorporated into the vector as a selectable marker in events where GFP selection was not possible.

[0050] The experiment was performed as follows. The blood-sucking Anopheles stepheni mosquito lays eggs 48-72 hours after sucking blood. Eggs were placed in Petri dishes containing a solution in isotonic buffer (150 mM NaCl, 5 mM KCl,...

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Abstract

A method for the genetic modification of an insect embryo, comprises first treating an insect egg under conditions which prevent or delay the hardening of the insect egg chorion, and then injecting a transposable element into the egg to permit integration of the element into the genome of the embryo. The method permits modifications to be made to mosquitoes, which may prevent transmission of a host parasite.

Description

field of invention [0001] The present invention relates to genetic manipulation of insects. In particular, the invention relates to genetic manipulation of mosquitoes. Background of the invention [0002] Malaria, the most important parasitic disease in the world today, is one of the major health threats in Africa, where 10% of the world's population suffers from more than 90% of the world's malaria infections. [0003] Malaria is caused by protozoan parasites of the genus Plasmodium. Of the four recognized human parasites (P. falciparum, P. vivax, P. ovale, and P. malariae), Plasmodium falciparum is the most dangerous and is the leading cause of mortality. [0004] The human malaria parasite is transmitted by mosquitoes of the genus Anopheles. At least 20 of the nearly 500 known types of Anopheles mosquitoes have been implicated in malaria transmission. In sub-Saharan Africa, transmission is mainly caused by three species of Anopheles, A. gamibiae, A. arabiensis, and A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033C12N5/10C12N15/09C12N15/85
CPCC12N15/85C12N2800/90C12N2830/00A01K67/033C12N2830/75C12N2840/20A01K67/0339C12N2830/002A61P11/00
Inventor A·克里森蒂C·弗莱米尼亚T·诺兰
Owner IMPLYX
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