105 results about "Insect egg" patented technology

In an aspect, a system for breeding and harvesting insects is provided and includes an egg-producing chamber structure configured to receive insect pupae for pupation and to permit emerged adult insects to mate and oviposit insect eggs, at least one oviposition region in the egg-producing chamber structure configured to receive the insect eggs and apertured to permit at least one of the insect eggs and neonates of the insect eggs to pass therethrough, at least one larvae-growth chamber in communication with the at least one oviposition region so as to be configured to receive the at least one of the insect eggs and neonates of the insect eggs, wherein the larvae-growth chamber is further configured to permit the at least one of the insect eggs and neonates of the insect eggs to transition into larvae and to hold feed material for the larvae, a harvesting receptacle positioned to hold larvae, and an inclined surface positioned to receive larvae from the at least one larvae-growth chamber, and to provide a passageway for the larvae to travel to the harvesting receptacle.

Embodiments of the present disclosure can provide an automated mass rearing system for insect larvae. The automated mass rearing system can facilitate hatching, feeding, monitoring the growth and emergence of insect larvae and pupae. In some embodiments, the automated mass rearing system can include a production unit, a transportation unit, a storage unit, a dispensing unit, and a monitoring unit. In some embodiments, this automated mass rearing system can facilitate mass mosquito growth from egg hatching all the way through to full adults or certain stages in between such as the larvae rearing process (i.e., from larvae to pupae) with little or no human intervention. By automating the rearing and transportation of insect eggs, larvae, and pupae, deaths or developmental issues can be minimized. Various techniques and apparatuses are used in this automation that causes minimal disturbance to the insects during development, and thereby maximizing survival rate and fitness of the insects.

A method for mass rearing of insects. A web including a plurality of wells is provided. Insect eggs or larvae and growth medium are loaded in the wells. The wells are covered. The web is rolled up. The eggs or larvae are incubated during eggs hatch and larval growth. The web is unrolled and the larvae harvested.

The invention discloses a multicomponent composite fertilizer special for peanuts, which belongs to a crop fertilizer. The multicomponent composite fertilizer special for peanuts comprises the following components: 35 percent of total nutrients including 16 percent of nitrogen, 10 percent of phosphorus and 9 percent potassium, more than or equal to 10 percent of medium and trace elements, 7-9 percent of organic matter and 2-5 percent of amino acid. The medium and trace elements comprise trace elements including molybdenum, zinc, manganese, boron and iron. The multicomponent composite fertilizer special for peanuts has the advantages of nutrient content assurance and reasonable proportioning balance. The multicomponent composite fertilizer special for peanuts can effectively improve the immunological competence of the peanuts, regulate endogenous hormone, inhibit the regeneration of insect eggs, activate the activity of root azotobacter, promote root development, quicken nutrient absorption, achieve the functions of strengthening seedlings, promoting growth and promoting flowering, prevent bacterial wilt, continuous cropping symptoms and leaf spots, also prevent excessive growth and take special effect on symptoms including mosaic disease, chlorotic disorder, flocculi, defoliation and the like. After applied, the multicomponent composite fertilizer special for peanuts can effectively improve the percentage of fertile fruits and the thousand grain weight, promote the mellow and full degrees of fruits and achieve remarkable effect of production increase.

The invention relates to a method for preparing herbarium specimens. The method comprises the following steps: (1) collecting plant specimens and routinely preprocessing the specimens; (2) preparing a Chinese herbal medicine solution; (3) scalding the preprocessed fresh specimens in the Chinese herbal medicine solution under 10-100 DEG C for 1 second to 10 minutes; (4) drying the scalded specimens in air or absorbing all water from the scalded specimens, and flatly pressing in a specimen clip; (5) carrying out low-temperature airing treatment on the prepared specimens for 5-160 hours at the temperature of 5-20 DEG C. According to the method, the Chinese herbal medicine solution is directly utilized to scald the fresh plant specimens, has very good effects of killing bacteria and insects and preventing molds, and can be used for killing insect eggs and spores attached to the surfaces of plants to ensure that the plants are relatively bright in color. In addition, the low-temperature airing treatment can ensure that the plants can keep the original color and are prevented from being mildewed, so that the specimens can be stored for a relatively long time by using the method.

The invention discloses a preparation method of a seaweed biological composite fertilizer. The preparation method of the seaweed biological composite fertilizer comprises the steps of preparing seaweed slag; preparing bacillus thuringiensis powder; and uniformly mixing the seaweed slag with the bacillus thuringiensis powder to obtain the seaweed biological composite fertilizer. The seaweed biological composite fertilizer comprises 300-600 parts by weight of the seaweed slag and 50-200 parts by weight of the bacillus thuringiensis powder. The prepared seaweed biological composite fertilizer ishigh in content of toxalbumin and high in Chinese chive maggot killing activity, does not have sick insect eggs, and has good effect of preventing and controlling Chinese chive maggots, and the application amount of chemical pesticides is reduced effectively. The seaweed biological composite fertilizer contains high content of active substances, is high in nutrient, can be decomposed by microorganisms in soil and can be absorbed and utilized by fragrant-flowered garlics, meanwhile, soil can be loosened, rooting and growth of the fragrant-flowered garlics can be promoted remarkably, nutrition required for growth is provided, the quality of the fragrant-flowered garlics is improved, the seaweed biological composite fertilizer is safe and healthful, environmental protection and zero pollutionis realized, and the seaweed biological composite fertilizer has quite good development prospect.

The invention belongs to the technical field of horticultural materials and equipment, and particularly relates to a preparation method of horticultural nutrient soil using ecological organic fertilizer. The method comprises the following steps of (1) organic fertilizer preparation, (2) planting base material preparation, (3) disinfection and insect pest preventionand (4) standing and maturation. The produced nutrient soil contains permeable soil medium such as medical stone, perlite and the like, the mature organic fertilizer which is formed by plant stalk and decomposing bacteria decomposing is used, and thus the soil is not easy to harden; through disinfection and insect pest killing, disinfection and insect pest prevention substances are added and mixed, and thus there is no insect egg and germin the soil. The horticultural nutrient soil can be applicable to the growth of various plants, in particular to horticultural flowers with poor resistance. Chemical fertilizer and pesticide need not to be added in the usage process, and accordingly the horticultural nutrient soil is quite green and environmentally friendly.

A timber preservative is formed by mixing cupric salt, dimethyl dodecyl benzyl ammonium chloride, allethrin and ammonia, wherein the cupric salt can undergo complexation and solidification with timber, thereby having a long-term poisonous effect on bacteria; the benzyl ammonium chloride is used as a bactericide; and the allethrin plays a role in deinsectization and killing insect eggs. Therefore, the timber preservative has excellent preservative effects and is avirulent to human body.

The invention relates to a method for establishing a cell line of insect eggs, which comprises the following steps: 1) dipping an insect oosperm sheet which has been laid for 90 to 100 hours in a sodium hypochlorite solution or a formaldehyde solution, and then disinfecting and washing the oosperm sheet; 2) pouring the treated eggs into an insect cell culture solution, and extruding the eggs one by one to release embryos; 3) cutting the embryos into tissue blocks to be cultured in an incubator; 4) adding the cell culture solution into the incubator to culture the tissue blocks continuously; 5) replacing the culture solution periodically until the proliferating cells are full of the whole culture bottle; 6) sucking out half of the cell culture solution containing newly proliferated single cells, putting the cell culture solution into a new culture bottle which is added with the same amount of a new cell culture solution, and putting the new culture bottle back to the incubator to culture the cells continuously; and 7) repeating the step 5) and the step 6) periodically to cause the cells to begin to passage so as to establish the cell line successfully, wherein the whole process is performed under a sterile condition. The insect eggs can be lepidoptera insect eggs. The method shortens the time from the prior 1.5 to 2 years to 1 to 3 months, thereby having quite obvious superiority and strong repeatability.

The present invention relates to a processing method of refined dried Brine shrimp Eggs. Said method includes the following steps: using 180-200 % sodium chloride aqueous solution and fresh water to separate and remove impurities at twice, then using 0.8% calcium hypochlorite solution as rinse solution to make rinsing, at the same time having the sterilizing action, and drying the Brine Shrimp Eggs to make its water content be 5-15%. Said invention has active separation procedure, uses air blast process to separate out dead eggs, then uses 5800-6200 LUX UV-ray lamp to make sterilization and disinfection, its Brine Shrimp Eggs hatching rate is 92-94%, the Brine Shrimp natality is 1:3.1-3.2, and its Brine Shrimp protein content is 52-54%.

The present invention relates to propagation process of breeding hepialus insect in arctic-alpine habitat. The process includes establishing hepialus raising farm in proper arctic-alpine habitat; controlling and reducing the natural enemy harm to hepialus; promoting the growth of herbage and brush plant to form rich vegetation root system for hepialus larva to take; throwing egg, larva or pupa of hepialus to increase the amount and density of hepialus; and collecting hepialus insect and hepialus egg in June to August. The collected hepialus egg is detected to have no entomogenous fungi carried or infected.

The invention discloses a composition for attracting hermetia illucens for concentrated oviposition and an application of the composition, and belongs to the technical field of insect breeding. The composition includes an attracting odor substance arranged on an oviposition substrate, and the attracting odor substance includes one or more selected from the group consisting of methyl acrylate, lactic acid, 3-methylindole, n-heptanoic acid and dimethyl disulfide, the odor substance having the attracting effect and oviposition substrate color having the attracting effect are combined to attract the hermetia illucens to crack channels of corrugated paper for oviposition, insect eggs are mainly concentrated in the corrugated paper, so that the oviposition is more concentrated, and the eggs areeasier to collect. The composition provided by the invention has the multiple combined effects of the odor substance, the oviposition substrate color and the crack channels, and can enhance the oviposition attracting effect on the hermetia illucens, thereby improving the concentrated oviposition and egg collection efficiency of the hermetia illucens.

The invention discloses a combined treatment method for livestock and poultry excrement through microbial preparations and insects. The method includes the following steps that the livestock and poultry excrement and conditioners are evenly mixed, the microbial preparations are inoculated, then the livestock and poultry excrement are composted and fermented, after primary composting, excrement compost is spread out and aired, and saprophytic insect eggs are inoculated for cultivation; after being mature, the insects are collected to feed bred animals, or the insects are dried, smashed and manufactured into a feeding protein source for use; then post-maturation is performed on the remaining excrement, and then the excrement is dried and smashed to be used as bio-organic fertilizers. The saprophytic insect eggs are inoculated into the excrement after fermentation, by the utilization of efficient transformation of the insects, the novel feeding protein source is developed, and the remaining excrement can be used as the bio-organic fertilizers after post-maturation processing. In this way, the problems that the livestock and poultry excrement pollutes the environments and stinks are solved, besides, insect protein can be harvested, the bio-organic fertilizers are produced, and the economic utilization value of fermentation treatment products of the livestock and poultry excrement is improved.

Methods of the disclosed precision guided sterile insect technique (pgSIT) include methods for directing male sexing in a genetically modified insect and methods of producing a progeny of geneticallymodified sterile male insect egg. These methods include integrating at least one nucleic acid sequence into a genome of a first insect, the at least one nucleic acid sequence having at least one firstguide polynucleotide targeting a female-essential genomic sequence that is required for female-specific viability, introducing an endonuclease into a second insect, and genetically crossing the firstinsect and the second insect thereby producing progeny expressing the endonuclease and the at least one nucleic acid sequence. For male sterility a second guide polynucleotide targets a male sterility genomic sequence that is required for male-specific sterility.

The invention discloses a high-efficient and environment-friendly treatment process of animal manure. The high-efficient and environment-friendly treatment process is characterized in that the treatment process comprises the following steps of: A) mixing raw juice pig manure with organic harmless filling material according to the proportion of (4-6): (6-4); B) adding substances after mixing into a treatment pot for performing fermentation treatment; C) heating to 70-90 DEG C in the treatment pot, performing oxygen-consuming fermentation for 12-24h, simultaneously adding and blending a high-temperature bacterial population, stirring to enable the temperature of contents to be uniform, enabling the bacterial population to be uniform and killing harmful bacteria and insect eggs by high temperature; D) switching off, maintaining at the temperature of 70-90 DEG C by relying on heat energy of fermenting organisms, and taking out of a tank after 12-24h; and E) accumulating after taking out of the pot, performing high-pressure air inflation, performing secondary fermentation to realize further loss of water, and fermenting for 12-24h so as to be used as an organic fertilizer for production. The process disclosed by the invention has the innovation points that: a traditional anaerobic way or an anaerobic and oxygen-consuming treatment mode is discarded, and the oxygen-consuming way is directly adopted; the treatment efficiency is high: as the high-temperature bacterial population is adopted and filled in the closed tank capable of being heated, the treatment efficiency is improved by dozens of times, and the harmless treatment can be performed at 24h; and the process is energy-saving and environment-friendly: in addition to initial heating, the high temperature required for fermentation relies on the heat energy which can be produced by the organisms, and the treatment result can be harmless completely.

Visual self-sucking method for inducing external gene into insect eggs is carried out by: under telescope, coating solution with external genes on insect eggs, pricking eggs to make external gene into eggs, making external genes combined with insect's gene to form transgenic insects. It makes technicians to do researches on transgenic insects with cheap instrument.

The invention relates to a box type insect incubator, belonging to the technical field of insect breeding and propagating equipment. The box type insect incubator is characterized by comprising hatching tanks and a box body, wherein plastic and foam plates with better heat-insulating performance are used as materials; the hatching tanks are fixed in the box body at intervals; upper and lower ends of the hatching tank are respectively blocked by heat-insulating ventilation cotton and warming and moisturizing cotton; and the bottom of the hatching tank is provided with an eggshell support, which provides supporting and needed proper temperature and humidity conditions for hatching of insect eggs while heating and moisturizing. A wall of the hatching tank is made of plastic and has better transparent effect, the overall hatching tank can be taken out when people need to observe, thus tremendous damage to the insect eggs caused by being in direct contact with the insect eggs is avoided. The box type insect incubator has simplicity in manufacturing, strong operability, low cost and obvious effect.

The invention relates to a fermentation bacterial agent for harmless treatment of solid organic matter and an application method of the fermentation bacterial agent. The fermentation agent is preparedfrom aspergillus oryzae, streptococcus thermophilus, bacillus thermophilus, streptococcus faecalis, bacillus mucilaginosus, biochar and auxiliary materials according to a certain proportion. Strainsused by the fermentation bacterial agent can correspondingly be obtained from separation of the natural environment, and have good symbiosis, and the antagonistic effect does not exist between the strains, functions, advantages and characteristics of each strain are fully used and applied to the harmless treatment of the solid organic matter, decomposition of feces can be accelerated, decomposition of organic matter is strengthened, pathogenic bacteria and insect eggs are killed through rapid heating, the odor of the feces is effectively eliminated, losses of ammonia nitrogen and other nutrients are reduced, fermentation time is shortened, and fermentation efficiency is improved, and products after fermentation and decomposition are high-quality raw materials for organic fertilizers; and the biochar is added on the basis of bacteria for the first time, the growth of microorganisms is greatlypromoted, and fermentation efficiency is improved.

A method for the genetic modification of an insect embryo, comprises first treating an insect egg under conditions which prevent or delay the hardening of the insect egg chorion, and then injecting a transposable element into the egg to permit integration of the element into the genome of the embryo. The method permits modifications to be made to mosquitoes, which may prevent transmission of a host parasite.

The invention discloses an insect-egg-and-pupa low-temperature drying device and a method for drying insect eggs and pupae thereof. The device comprises a box body, blades and a mesh tube, a refrigerating tube is installed on the left wall of the interior of the box body, a separating net is fixed to the interior of the box body, the blades and a driving motor are mutually connected through a connecting shaft below, a driving motor is installed on the right surface of the interior of the interior of the box body, the mesh tube and a rotary shaft are mutually connected through a supporting rodarranged at a left end port, scattering blades are arranged on the outer side of the rotary shaft, and a feeding hopper is installed on the upper surface of the box body. The method comprises the steps that wet eggs (pupae) are placed in the box body, the driving motor is started, and the mesh tube is driven to rotate by the driving motor to perform low-temperature drying on the wet eggs. According to the device and the method, the problems of interior or even surface rapid moisture losing of eggs and pupae on a surface layer caused by rapid air drying at room temperature or high temperature,poor survival of insects, reduced production suitability and the like can be prevented.

The invention discloses a method for preparing a slow-release biological fertilizer and relates to the technical field of preparation of biological fertilizers with special functions. The method comprises the following steps: mixing dead animal bodies, soybean cakes and animal excrement, and composting so as to obtain a mixture A; crushing oil-tea camellia bran, pine needles, persimmon tree leaves, clove tree leaves, orange peel and tobacco leaves, and composting so as to obtain a mixture B; uniformly mixing the mixture A with the mixture B, and pelletizing. According to the slow-release biological fertilizer prepared by using the method disclosed by the invention, organic matters are not completely decomposed after incomplete composting and fermentation, a fermentation process can be slowly started after the fertilizer is applied into soil, and no chemical substance which cannot be recycled or degraded is used in the fertilizer, so that soil pollution can be avoided, the process is simple, and the cost is low; meanwhile, the fertilizer contains multiple trace gentle biological sterilization substances and insect killing and expelling substances, so that both the requirements of crop growth on nutrition are met and a part of harmful bacteria in the organic matter decomposition process can be killed, and insect eggs or harmful insects can be killed and expelled.

The invention discloses a radix stephaniae tetrandrae storage method which comprises steps of cleaning, slicing, drying, packaging, sterilization, storage, and the like. By adopting the radix stephaniae tetrandrae storage method, ethylene oxide is adopted for sterilization, and molds and insect eggs which can be carried by medicinal materials can be killed; through hydrogen peroxide fumigation, awarehouse can be sterilized; through regular ultraviolet lamp radiation, and strict control on temperature and humidity environments of the warehouse, growth of molds can be very well inhibited by thecomprehensive measures, and radix stephaniae tetrandrae can be effectively prevented from molds in the storage process; through a unique safe and pollution-free traditional Chinese medicine insect expelling bags, insects can be effectively expelled, and the radix stephaniae tetrandrae can be effectively prevented from worm damage in the storage process; due to a relatively low temperature dry environment in the warehouse, effective components of the radix stephaniae tetrandrae can be maintained, and a medicinal effect can be maintained to the maximum extent.

The present invention relates to hatching of insect egg to obtain live fish feed, and is especially the hatching process of Branchinella kugennumaensis egg. The hatching process includes the following steps: pre-treatment of soaking wet Branchinella kugennumaensis egg in alkali solution for 0.5-2 hr and water rinsing; setting the egg in 20-40 ppm KMnO4 solution in 3-8 g egg / L at 25-30 deg.c, and continuously illuminating in the light intensity over 1000 Lux while inflating to hatch the egg. In the process, egg is hitched in 24-48 hr into nauplius. The present invention provides great amount of nauplius for fresh water aquaculture.

The present invention relates to a method of controlling insect infestation whereby an insecticidal coating having an extended residual period of a few months to a year is applied to a surface. A removable cover may be positioned near the surface to create a substantially darkened region to exploit some insects' natural tendencies to hide in dark places during daylight hours. Alternatively, the insecticide may be applied prior to the hatching of the insect eggs to control the population upon hatching. The method is especially effective for controlling the presence of gypsy moth caterpillars on trees. The present method may be accomplished by covering a portion of a tree trunk with an insecticidal coating containing an active ingredient such as chlorpyrifos, pyrethroid, permethrin, or tralomethrin and covering the coated area with burlap. In addition, the method of the present invention can be used to control the presence of insects on the sides of houses or other structures.

Disclosed is a pet comb apparatus. The pet comb apparatus includes an elongated shaft, an illumination system, and at least one row of a plurality of teeth extending outwardly and perpendicularly from the elongated shaft. The light from the illumination is used to locate fleas, ticks, insect eggs, skin infections and injuries on a pet.

The invention discloses a preparation method and nucleic acid construct of male sterile lepidoptera insects. The method comprises the steps that 1, the Ser2 gene knockout nucleic acid construct is built, wherein the nucleic acid construct comprises the following operable connection elements from the 5' terminal to the 3'terminal, and the operable connection elements include one U6 promoter, a first Ser2 genetic target, polyA, another U6 promoter, a second Ser2 genetic target and polyA; 2, the construct and a PHA3PIG plasmid capable of expressing Piggybac transposase are used for co-transformation of fresh lepidoptera insect eggs, a generation G0 is obtained and subjected to selfing, and a generation G1 is obtained; 3, the generation G1 and transgenic lepidoptera insects for expressing Cas9protein are mated to obtain a generation G2. According to the method, a CRISPR / Cas9 technology based on a PiggyBac transposon is used for successfully establishing the male sterile lepidoptera insects on the premise that normal mating behaviors are not affected, and the method has important value in prevention and treatment of lepidoptera pests through the male sterility technology.

The invention belongs to the technical field of animal nutritional additives and particularly discloses an animal nutrition enhancer which is prepared from edible insects eggs. Further, the edible insects eggs are one or more of black soldier flies eggs, tenebrio molitors eggs, flies eggs and the like. The invention discovers that the insects eggs have better effects of enhancing nutrition, promoting growth and health care to animals, in particular to primary animals, weanling animals, animals with weak physique or animals with diseases. The provided animal nutrition enhancer is simple in preparation and convenient to use. According to the animal nutrition enhancer, taking the edible insect eggs as main components of the nutrition enhancer, the health level of the animals including livestock, aquatic animals and the like can be effectively increased, and the feed intake and the feed conversion level can also be increased, so that the animal nutrition enhancer increases comprehensive benefits of cultivation and has a good application prospect.

The invention relates to the technical field of vegetable introduction planting, and discloses a greenhouse planting method of wild chive. The method comprises greenhouse establishing, chive processing, chive planting, chive managing, common sage herb planting and joint management; the yield and quality of the wild chive are significantly improved, the annual acre yield of the wild chive reaches 6439 kg, and the annual acre yield of common sage herb reaches 1127 kg; leaves and fibrous roots are cut, the generation of newly borne leaves and the fibrous roots is promoted to prevent the slow germination of the leaves and roots after the transplanting is conducted due to the fact that the leaves and the fibrous roots do not adapt to a greenhouse environment, high-temperature short-time processing is conducted after plant division is conducted to kill residual pathogenic bacteria and insect eggs on the roots, stimulation on root cells is generated to enhance the environmental adaptation capability and cell division capability, the chive is placed in root promoting liquid to be soaked immediately after high-temperature processing is conducted to prevent the deterioration and rotten of cells at the incision positions of the fibrous roots, the activity of the cells is improved to accelerate the generating and growing of the fibrous roots, and the rooting percentage of the wild chive is improved.