Hydrolytic extrating method for medicinal component of pilose antler
A technology of medicinal ingredients and extraction methods, which is applied in the field of extracting active ingredients of traditional Chinese medicine, can solve the problems of velvet antler resource waste and achieve the effect of convenient application
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Embodiment 1
[0012] Take a certain amount of fresh antler 1kg, add 3000ml of phosphate buffer, grind it with a tissue grinder, add biocatalysts: pepsin 10g, activator zinc chloride 0.1g, adjust the pH value to 1.5, and adjust the temperature of the solution Adjust the temperature to 25°C, put it into the reaction tank for 4 hours, centrifuge to remove the residue, and collect 3200ml of transparent and clear liquid.
Embodiment 2
[0014] Take a certain amount of fresh velvet antler 1kg, add 3000ml of citrate buffer, grind it with a tissue grinder, add biocatalysts: 8g of papain, 0.08g of activator calcium chloride, adjust the pH value to 7.0, and mix the solution Adjust the temperature to 37°C, put it into the reaction tank for 5 hours, centrifuge to remove the residue, and collect 3300ml of transparent and clear liquid.
Embodiment 3
[0016] Take a certain amount of fresh velvet antler 1kg, add 3000ml of lactate buffer, grind it with a tissue grinder, add biocatalysts: 10g of pancreatin, 0.15g of activator manganese chloride, adjust the pH value to 9.0, and mix the solution with Adjust the temperature to 52°C, put it into the reaction tank for 3 hours, centrifuge to remove the residue, and collect 3340ml of transparent and clear liquid.
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