Root inductive method for microbody reproduction of Japan dahurian larch
A technology for micropropagation and larch, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of no relevant reports on the micropropagation of Japanese larch, only focusing on scientific problems, low reproduction rate, etc. The effect of promoting the recovery of juvenile growth characteristics, fast reproduction speed, and high reproduction efficiency
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Embodiment 1
[0029] The clonal plants of Japanese larch were selected, and the one-year-old branches before the buds germinated in early spring were used as materials. After the stem induction was completed, the improved MS medium was selected, and the method of combining variable temperature culture and subculture was used to achieve Root induction of Japanese larch micropropagation; specific operations are as follows:
[0030] 1. Material collection and pretreatment
[0031] The Japanese larch No. 38 clone was selected from the seed garden of Wulong Forest Farm, Fushun County, Liaoning Province. The tree was 10 years old, and the upper branches of the 1-year-old tree crown were collected. The collection time was before the buds germinated in early spring. Brown or dark brown turns into shiny light brown or light brown. Store at 4°C. Take it out and cultivate it in water at room temperature for about two weeks until the buds are bright.
[0032] 2. Medium Preparation
[0033] 1) Prepare...
Embodiment 2
[0046] The difference from Example 1 is:
[0047] Select the 28-year-old Japanese larch clone Chao No. 6 plant, collect the 1-year-old branches with full winter buds in March, and use the adventitious stems with induced elongation greater than 2cm as materials;
[0048] Prepare rooting medium, add appropriate amount of NAA and AC to the improved MS medium, the added concentrations are: NAA 0.3mg / l, AC 1.0g / l;
[0049] Move the Erlenmeyer flask inoculated with adventitious stems in step 4 to 28°C for constant temperature and dark light culture for 5 days, then under the condition of light intensity 3,000Lux, subculture for a total of 70 days, the light-dark cycle is 14 / 10 hours, subculture 3 times, as a result, 5 of the 25 explants took root, and the plant regeneration was completed. Figure 2C , 2D The regenerated plants in this example are shown, showing that the rooting induction process can also be completed by using NAA with an appropriate concentration in the present in...
Embodiment 3
[0051] Carry out the test of embodiment 1 and embodiment 2 repeatedly, and condition is with above-mentioned respectively. Figure 3A , 3B , 3C, 3D show the results of the above-mentioned two embodiment repeated tests, wherein Figure 3A Be the rooting medium of present embodiment 1, Figure 3B with 3C For the verification result of Example 1, Figure 3D It is the verification result of Example 2.
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