Recombination of human soluble TRAIL protein, the preparing method and the application in preparing antineoplastic medicine

A soluble protein technology, applied in the direction of recombinant DNA technology, botany equipment and methods, biochemical equipment and methods, etc., can solve the problem of normal cell killing
CN1500808AInactive Publication Date: 2004-06-02ๆŽๅฎ
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
ๆŽๅฎ
Publication Date
2004-06-02
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The present invention discloses recombinant soluble human tumore necrosis factor related death inducing ligand and its preparation process and application in preparing antitumor medicine. The present invention adopts human tonsil tissue mRNA as template to amplify TRAIL coding sequence, constitute engineering colibacillus for expressing rhsTRAIL and engineering saccharomycete, establish the rhsTRAIL engineering colibacillus fermentation, occlusion body washing and destination protein purification process and the engineering saccharomycete and destination protein purification process separately, and obtain pure rhsTRAIL product, which has molecular weight of 19.6-24 KD and exists in both monomer and dimmer. The rhsTRAIL can induce death of several kinds of cancer cells outside body, inhibit amplification of liver cancer cell in tumor loading mouse obviously and kill tumor cell selectively, so that it may be used in preparing effective antitumor medicine.
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Description

field of invention

[0001] The present invention relates to recombinant human soluble TRAIL protein, its preparation method and its application in the preparation of anti-tumor drugs, specifically, the present invention relates to tumor necrosis factor-related apoptosis ligand (hereinafter referred to as TRAIL protein) gene cloning, The preparation method and its application in the preparation of anti-tumor drugs, the TRAIL protein has the effect of inducing tumor cell apoptosis in vitro and anti-tumor in vivo, can be used for the treatment of various tumors, and has potential application value in the treatment of clinical tumors . Background technique

[0002] At present, the morbidity and mortality of malignant tumors are still increasing year by year, becoming the first or second fatal disease. The treatment of tumor mainly depends on the three major therapies of radiotherapy, chemotherapy and surgical resection. Among them, surgical resection has its limitations, while ...

Claims

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