Method for constituting TLC finger-print pyrogram of hypericum-japonicum medicinal herbs and its standard finger-print pyrogram

A technology of fingerprint spectrum and construction method, which can be used in material separation, analysis materials, measuring devices, etc., and can solve problems such as incomplete quality standards of Tian Jihuang.

Inactive Publication Date: 2004-09-15
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the current quality standard of Tian Jihuang is extremely imperfect.

Method used

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  • Method for constituting TLC finger-print pyrogram of hypericum-japonicum medicinal herbs and its standard finger-print pyrogram
  • Method for constituting TLC finger-print pyrogram of hypericum-japonicum medicinal herbs and its standard finger-print pyrogram
  • Method for constituting TLC finger-print pyrogram of hypericum-japonicum medicinal herbs and its standard finger-print pyrogram

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] Embodiments of the present invention are as follows:

[0028] 1. Instruments and reagents

[0029] 1.1 Instruments: Automatic TLC Sampler 4 (CAMAG, Switzerland); TLC camera (Reprostar 3, CAMAG, Switzerland); TLC Scanner (TLC Scanner 3, CAMAG, Switzerland); double-slot expansion cylinder (Switzerland CAMAG); polyamide film 10cm×20cm (Yineng Chromatography Material Factory).

[0030] 1.2 Test drug: a total of 32 batches of Tianjihuang medicinal materials were collected from Jiangsu, Zhejiang, Yunnan, Sichuan, Guangdong, Guangxi, Guizhou, Hunan, Jiangxi, Fujian and other places. All samples were identified as specified in the "Chinese Pharmacopoeia" The variety is the whole herb of Hypericum japonicum Thunb. The reference substance was quercitrin (WA17779, Fluka Company); all the reagents used in the experiment were of analytical grade.

[0031] 2. Methods and Results

[0032] 2.1 Preparation of the test solution: Remove the weeds in the whole herb of Tianjihuang, wash...

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Abstract

The invention comprises a series of preparing steps as following: taking powder of Japanese St.Johnswort, adding water, reflow distilling, filtering, decompression concentrating, adding alcohol, homogenizing, standing overnight, filtering, retrieving almost all of alcohol from filtrate, adding alcohol to dissolve the densed filtrate and displacing it to 10ml volumetric flask, constant volume to a certain scale so as to obtain solution of sample; putting weighed up quercetin with accuracy into a volumetric flask, adding 95% alcohol to dissolve quercetin and dilute it to a certain scale, shaking it up so as to obtain 1 mg / ml referenced solution. Then, thin-layer chromatography and scanning are carried out in following condition: polyamide thin film, specific developing agent and color developing reagent, 320nm scanning wavelength so as to obtain TLC fingerprint. Analyzing 32 batches of Japanese St.Johnswort constructs standard fingerprint, which is applied to quality control.

Description

technical field [0001] The invention relates to a method for constructing the TLC fingerprint of Tianjihuang medicinal material and the standard fingerprint of Tianjihuang medicinal material obtained by the method. Background technique [0002] The research on the quality standardization of traditional Chinese medicine and its quality evaluation method is an urgent problem to be solved in my country's traditional Chinese medicine industry. Due to various reasons, the current quality standards of traditional Chinese medicine are not perfect, and confused or even counterfeit products of traditional Chinese medicine are often seen in the market, seriously endangering people's medication safety. In order to control the spread of fake and inferior medicinal materials, it is necessary to strengthen the research on the quality of traditional Chinese medicine and formulate a more perfect, more accurate and more authoritative method for evaluating the quality of traditional Chinese m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86G01N30/90
Inventor 苏薇薇陈丽云吴忠王永刚彭维高燕
Owner SUN YAT SEN UNIV
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