New type automatic malignent fumour auxiliary diagnostic instrument

A malignant tumor and diagnostic instrument technology, which is applied in the field of malignant tumor diagnostic instruments, can solve the problems of tedious nucleosides and time-consuming, and achieve the effects of less sample volume, improved precision, and reduced analysis time

Inactive Publication Date: 2004-10-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Due to the complexity of the sample matrix, RP-HPLC analysis of nucleosides in urine involves tedious and time-consuming offline sample pretreatment process

Method used

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  • New type automatic malignent fumour auxiliary diagnostic instrument
  • New type automatic malignent fumour auxiliary diagnostic instrument
  • New type automatic malignent fumour auxiliary diagnostic instrument

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Embodiment Construction

[0031] The instrument includes the following components: three high-pressure pumps, an automatic sampler, a UV-visible detector, a six-way valve, and a chromatographic workstation (affinity chromatographic column and analytical column) for data collection. The flow chart is as follows: figure 1 .

[0032] Since there are tens of thousands of chemical substances in urine, the content of nucleosides is very low, and there is too much interference from other components, it is impossible to directly analyze the samples; most literatures use phenylboronic acid affinity chromatography to pre-separate and enrich nucleosides in urine (see literature 2), this step is cumbersome, time-consuming and error-prone. The sample pretreatment process of the present invention only needs to adjust the pH of the collected urine sample with ammonia water, centrifuge to remove protein, take the supernatant and add the internal standard Br8G, then the sample can be directly analyzed.

[0033] On the...

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Abstract

An automatic auxiliary diagnosing instrument for cancer is composed of high-pressure pumps A, B and C, automatic specimen feeder, 6-way valve, affinity chromatographic column, mixer, analyzing column, and ultraviolet-visual light tester. It can automatically separate the nucleoside from urine and analyze it for diagnosing cancers. Its advantages are high accuracy, less needed speciment and high speed.

Description

technical field [0001] The invention relates to a diagnostic instrument for malignant tumors, in particular to a novel automatic auxiliary diagnostic instrument for malignant tumors. Background technique [0002] Malignant tumor (cancer) is one of the common malignant diseases. There are about 6 million newly diagnosed cases in the Golden World every year, accounting for the second place in the death rate of various diseases; in my country from 1990 to 1992, there were 108.26 deaths per 100,000 people Malignant tumors accounted for 17.94% of the total death toll; by 1994, this figure had risen to 22.05%, and this trend had not been effectively suppressed; now, there are about 1.6 million new tumors in my country every year. According to the modern medical level, malignant tumors are not incurable, but difficult to detect early. If early diagnosis can be realized, more than 60% to 90% of patients can be cured. [0003] Cancer is a very complex disease. It is caused by multipl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/36G01N30/40G01N33/574
Inventor 许国旺郑育芳孔宏伟逄涛杨军
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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