Notoginseng adventitious root tissue culture and its production method

A production method and tissue culture technology, applied in the field of tissue culture and production of Panax notoginseng adventitious root, can solve problems such as retention, achieve short growth time, solve natural condition restrictions, and achieve significant economic and social benefits

Inactive Publication Date: 2005-01-19
高文远 +1
View PDF0 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the work on the tissue culture of medicinal plants in my country stays at the stage of petri dishes and shake flasks. So far, the in vitro induction and cultivation of Panax not

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0008] Adjust 100mL MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under high pressure for 15 minutes for later use; select Panax notoginseng roots that grow well and are free from diseases and insect pests, rinse and disinfect with tap water, distilled water and detergent for 10 minutes, then wash with distilled water, soak in 70% ethanol for 3 minutes, and then use Disinfect with 1% sodium hypochlorite (add a few drops of surfactant Tween-20) for 30 minutes, and finally wash with distilled water for 3-5 times to obtain aseptic notoginseng roots. Cut the sterilized roots of notoginseng into small pieces with a sterilized scalpel, inoculate them on the MS solid medium prepared in advance, and induce the callus of notoginseng at 25°C and in the dark, and culture them for 4 weeks Visible callus formation. Adjust 100mL MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under pressure for 15 minutes and se...

Embodiment 2

[0010] Adjust 100mL 1 / 2MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under high pressure for 15 minutes for later use; select Panax notoginseng roots that grow well and are free from diseases and insect pests, rinse and disinfect with tap water, distilled water and detergent for 10 minutes, then wash with distilled water, soak in 70% ethanol for 3 minutes, and then use Disinfect with 1% sodium hypochlorite (add a few drops of surfactant Tween-20) for 30 minutes, and finally wash with distilled water for 3-5 times to obtain aseptic notoginseng roots. Cut the sterilized notoginseng roots into small pieces with a sterilized scalpel, inoculate them on the 1 / 2MS solid medium prepared in advance, and induce the notoginseng callus at 24°C and dark conditions, and culture for 5 Callus formation can be seen after one week. Adjust 100mL 1 / 2MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under pressure for 15...

Embodiment 3

[0012] Adjust 100mL 3 / 4MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm-2 Sterilize under high pressure for 15 minutes for later use; select Panax notoginseng roots that grow well and are free from diseases and insect pests, rinse and disinfect with tap water, distilled water and detergent for 10 minutes, then wash with distilled water, soak in 70% ethanol for 3 minutes, and then use Disinfect with 1% sodium hypochlorite (add a few drops of surfactant Tween-20) for 30 minutes, and finally wash with distilled water for 3-5 times to obtain aseptic notoginseng roots. Cut the sterilized notoginseng roots into small pieces with a sterilized scalpel, inoculate them on the prepared 3 / 4MS solid medium, induce the notoginseng callus at 25°C and dark conditions, and culture for 5 Callus formation can be seen after one week. Adjust 100mL 3 / 4MS solid medium to pH 6.0 with HCl and NaOH solution, at 121°C, 1.2kg / cm -2 Sterilize under pressure for 15 minutes and set ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a notoginseng adventitious root tissue culture and its production method wherein the method comprises, notoginseng callus inducement, notoginseng callus expansion and successive transfer culturing, notoginseng adventitious root inducement, notoginseng adventitious root expansion and successive transfer culturing, screen selecting and active component determination and expansion for different root systems of notoginseng adventitious roots, and reactor culturing of notoginseng adventitious root.

Description

technical field [0001] The invention relates to a tissue culture and production method of adventitious roots of plants, in particular to a tissue culture and production method of notoginseng adventitious roots. Background technique [0002] Panax notoginseng is one of the most commonly used Chinese medicinal materials in my country, and it is also a best-selling herbal medicine in the international market. It can be directly eaten as a tonic, and it is also an important raw material for many health products and medicines, such as Xuesaitong, Yunnan Baiyao and Danshen dripping pills. Due to the limitation of geographical conditions, the cultivation area of ​​this medicinal material is limited, and it is mainly distributed in a narrow area around Wenshan Prefecture, Yunnan Province, my country, and it is also affected by "the cultivated land cannot be reused". Since the problem of "old seven land" cannot be solved, the land for cultivating Panax notoginseng can only be expand...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 高文远贾伟
Owner 高文远
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap