Gene cloning of polyepitope antigen of hepatitis C virus and its coding sequence
A hepatitis C virus, coding sequence technology, applied in anti-receptor/cell surface antigen/cell surface determinant immunoglobulin, gene therapy, genetic engineering and other directions, can solve the problem of unsatisfactory effect and cannot be used for hepatitis C vaccine preparation , the epitope is not ideal, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0027] The specific construction method of hepatitis C virus multi-epitope antigen gene clone (hcvme) is as follows:
[0028] 1. Synthesizing 26 oligonucleotide fragments required for the hcvme gene (see SEQ ID NO.2, synthesized by Shanghai Shenergy Gambling Biotechnology Co., Ltd.).
[0029] 2. The 26 oligonucleotide fragments are both primers and templates. The two gene fragments A and B before and after the PCR reaction are respectively synthesized. Both PCR synthesis and splicing reactions use Expand TM Long Template PCR System.
[0030] 1. The synthesis of gene fragment A in the front of hcvme ( figure 2 )
[0031] 0.5 μl each of oligonucleotide fragments 1-14 (30 μM)
[0032] dNTP (10mM) 2.0μl
[0033] 10×PCR buffer 5.0μl
[0034] DNA polymerase mixture (10 units / μl) 1.0μl
[0035] Sterilized redistilled water 35μl
[0036] The PCR reaction was performed under the following conditions: 35 cycles of 94°C for 30 seconds, 65°C for 30 seconds, and 74°C for 1 minute,...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com