Relativity between gene of endothelin 1 and essential hypertension

A vasoconstriction and gene technology, applied in the fields of molecular biology and medicine, can solve problems such as the unconfirmed correlation between EDN1 gene SNP and essential hypertension, and the unconfirmed report of the correlation between EDN1 gene and essential hypertension

Inactive Publication Date: 2005-08-31
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Although there have been many studies on the relationship between various gene polymorphisms and essential hypertension, there is no report confirming the correl

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1.1 Research objects

[0069] Since blood pressure is a quantitative trait that involves multiple genes and is affected by a variety of environmental factors, genetic heterogeneity must be considered when analyzing related genes. Selecting isolated populations with relatively simple genetic backgrounds as materials for the study of polygenic diseases will help reduce the impact of genetic heterogeneity. At the same time, because these populations have relatively fixed living environments and relatively consistent living habits, the influence of environmental factors is greatly affected. reduce. Selecting such a population, whether it is used for linkage disequilibrium analysis or selecting families from it for family analysis, will help to improve the detection sensitivity of hypertension susceptibility loci. Therefore, in this example, the isolated population is selected as the research object (the isolated population has the advantages of relatively consistent geneti...

Embodiment 2

[0089] Essential Hypertension Susceptibility Test Kit

[0090] As described in Example 1, the mutation at position 10868 C→T in SEQ ID NO: 1 is closely related to hypertensive disease. Therefore, EDN1 gene-specific primers can be designed based on this mutation for detection using the patient's DNA as a template for amplification.

[0091] name

sequence

concentration

sense primer

SEQ ID NO: 2

100pmol

Antisense primer

SEQ ID NO: 3

100pmol

PCR reaction solution

Taq enzyme dNTP magnesium ion PCR reaction buffer

[0092] 3ml of blood is drawn from the male patient to be tested, and DNA is extracted from the blood using conventional methods (or using a specific kit). Dilute the PCR primers in the hypertension detection kit to 1μmol / μl, and use the extracted DNA as a template to perform a PCR reaction with the provided primers. After purification of the PCR product, ABI-PRISM TM 377 DNA sequencer was used for bidire...

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PUM

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Abstract

A process for testing the susceptibility of primary hypertension includes detecting if there are variations in endotheline (gene EDN1, transcript and protein of an individual and determining its high susceptibility if there are. Its reagent kit is also disclosed.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it relates to the single nucleotide polymorphism (SNP) of endothelin 1 gene (Endothelin 1, EDN1) and its correlation with essential hypertension. The present invention also relates to methods and kits for detecting these SNPs. Background technique [0002] Hypertension refers to a group of clinical syndromes with elevated systolic or diastolic blood pressure. Elevated blood pressure has obvious causal relationship with coronary heart disease, renal dysfunction, hypertensive heart disease and hypertension complicated by stroke. The latest diagnostic criteria for hypertension are systolic blood pressure ≥19kpa (140mmHg) or diastolic blood pressure ≥12kpa (90mmHg). [0003] Most hypertensive patients only have mild symptoms in the early stage of elevated blood pressure, such as headache, dizziness, insomnia, tinnitus, irritability, difficulty concentrating...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68
Inventor 金力黄薇姜正文王颖张晨辉李艳平王志敏肖君华卢大儒
Owner FUDAN UNIV
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