Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Antibody diagnosing reagent kit for detecting HepatitisType C virus, its preparation and method for detection

A diagnostic kit and hepatitis C virus technology, applied in the direction of measuring devices, material analysis by observing the impact on chemical indicators, instruments, etc., can solve the problem of only detecting IgG antibodies, etc., to reduce the probability of HCV infection, The effect of shortening the window period and improving the sensitivity and accuracy

Inactive Publication Date: 2005-09-21
北京科卫临床诊断试剂有限公司
View PDF0 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of this invention is to provide a kind of diagnostic kit for detecting hepatitis C virus antibody and its preparation method and detection method, which can solve the technical problem that the prior art can only detect IgG antibody; Issues to shorten the window period and further improve detection sensitivity and specificity

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibody diagnosing reagent kit for detecting HepatitisType C virus, its preparation and method for detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] [1], the present invention detects the hepatitis C virus antibody diagnostic kit component embodiment:

[0040] (1), pre-coated enzyme-linked plate coated with HCV antigen;

[0041] (2), use horseradish peroxidase-labeled enzyme to label HCV antigen conjugate;

[0042] (3), the washing liquid that contains the phosphate buffered saline of Tween;

[0043] (4), color developing solution A of citrate buffer containing hydrogen peroxide;

[0044] (5), color developing solution B of citrate buffer containing tetramethylbenzidine;

[0045] (6), stop solution containing 2M sulfuric acid solution;

[0046] (7), the sample diluent of the phosphate buffered saline solution containing casein;

[0047] (8), negative control normal human serum;

[0048] (9), the positive control contains the human serum of HCV antibody.

[0049] [2], the present invention detects the preparation method embodiment of hepatitis C virus antibody diagnostic kit:

[0050] I, the preparation of the...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

This invention relates to hepatitis C virus diagnose agent box and its process method, which comprises the following steps: joining HCV regroup antigen to the buffer liquid into the enzyme yoke plate; hatching overnight for buffer liquid washing enzyme yoke plate; then joining buffer liquid and after hatching to abandon hole liquid for total enzyme yoke plate to complete the process of yoke plate; combining the marked HCV regroup antigen and horse radish peroxides enzyme through improved iodic acid method to complete the enzyme marked HCV antigen. The agent box has color liquid A and B, terminal liquid, sample diluent, negative comparing normal serum, positive comparing human serum with HCV antibody.

Description

(1) Technical field [0001] The invention relates to a diagnostic kit for detecting hepatitis C virus antibody, a preparation method and a detection method thereof. (2) Background technology [0002] Hepatitis C virus (HCV) belongs to the Flaviviridae family and is a non-A and non-B hepatitis virus associated with blood transfusions discovered in 1989. Hepatitis C is a global epidemic. According to the statistics of the World Health Organization, the global HCV infection rate is about 3%. It is estimated that about 170 million people are infected with HCV, and there are about 35,000 new cases of hepatitis C every year. National serological epidemiological survey data show that the anti-HCV positive rate of the general population in my country is 3.2%. HCV is mainly transmitted by blood, including: (1) Transmission by blood transfusion and blood products. (2) Spread through damaged skin and mucous membranes. (3) Mother-to-child transmission. HCV is a common cause of cirrho...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/78G01N33/535G01N33/543G01N33/569
Inventor 王保君冯长访马晓晖
Owner 北京科卫临床诊断试剂有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com