Angelica sinensis and ligusticum wallichii Chinese medicinal crop and establishing method for its preparation finger print and standard fingerprint

A technology of standard fingerprints and establishment methods, which is applied in the direction of material separation, material analysis, measuring devices, etc., can solve the problems of no reports on fingerprint research, and achieve the effects of reducing risks, saving costs and time, and controlling comprehensively

Inactive Publication Date: 2005-12-14
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the HPLC fingerprint of Angelica sinensis and its compound preparations

Method used

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  • Angelica sinensis and ligusticum wallichii Chinese medicinal crop and establishing method for its preparation finger print and standard fingerprint
  • Angelica sinensis and ligusticum wallichii Chinese medicinal crop and establishing method for its preparation finger print and standard fingerprint
  • Angelica sinensis and ligusticum wallichii Chinese medicinal crop and establishing method for its preparation finger print and standard fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The establishment of implementation example 1 angelica standard fingerprint

[0039] 1. Instruments and reagents

[0040] SPD-M10A VP High Performance Liquid Chromatograph (Shimadzu Corporation); SPD-M10A VP Diode Array Detector (Shimadzu Corporation); CTO-10AS VP Column Thermostat (Shimadzu Corporation); 98-1-B Type electronic thermostat heating mantle (Tianjin Test Instrument Co., Ltd.); FW-100 high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.).

[0041] Ligustilide reference substance (self-made, purity over 98%); Angelica decoction pieces purchased from Tianjin Traditional Chinese Medicine Decoction Pieces Factory; methanol is chromatographically pure, and other reagents are analytically pure.

[0042] 2. Chromatographic conditions

[0043] Chromatographic column: ODS C 18 Chromatographic analysis column (4.6×200mm, 5μm, Dima Company); mobile phase A: methanol-HOAC (99.6:0.4), mobile phase B; water-HOAC (99.6:0.4); gradient elution, the elution p...

Embodiment 2

[0062] Implementation Example 2 Establishment of Standard Fingerprint of Rhizoma Chuanxiong

[0063] Based on the establishment of the fingerprints of Angelica medicinal materials in Implementation Example 1, the standard fingerprints of Rhizoma Chuanxiong medicinal materials were established. The instruments and reagents, chromatographic conditions, and fingerprint preparation methods were all the same as in Example 1. The precision inspection and reproducibility inspection of the method all meet the technical requirements of fingerprint research, and the solution is stable within 24 hours. The difference from Example 1 lies in the determination of common peaks. Through the mensuration of 10 batches of Rhizoma Chuanxiong medicinal material fingerprint chromatograms, compare its chromatogram, determine total peak 17, take No. 16 peak (ligustilide peak) as reference thing and exceed total peak area 10% common peak is No. 6 peak (A Ferulic acid, RT 0.373), No. 12 peak (RT 0.689...

Embodiment 3

[0069] Implementation Example 3 Establishment of Standard Fingerprint of Compound Angelica Chuanxiong Injection

[0070] 1. Instruments and reagents

[0071] SPD-M10A VP High Performance Liquid Chromatograph (Shimadzu Corporation); SPD-M10A VP Diode Array Detector (Shimadzu Corporation); CTO-10AS VP Column Thermostat (Shimadzu Corporation); 98-1-B Type electronic thermostat heating mantle (Tianjin Test Instrument Co., Ltd.); FW-100 high-speed universal pulverizer (Tianjin Test Instrument Co., Ltd.).

[0072] Ligustilide reference substance (self-made, purity above 98%); Angelica Chuanxiong decoction pieces were purchased from Tianjin Traditional Chinese Medicine Decoction Factory; Chromatographically pure, the rest of the reagents were analytically pure.

[0073] 2. Chromatographic conditions

[0074] Chromatographic column: ODS C 18 Chromatographic analysis column (4.6 × 200mm, 5 μm, Dima Company); mobile phase A: methanol-HOAC (99.6: 0.4), mobile phase B: water-HOAC (99....

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PUM

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Abstract

The present invention relates to fingerprint establishing method and standard fingerprint of angelica and Chuanxiong rhizome and their compound preparation. The standard fingerprint establishing method includes: extracting angelica and/or Chuanxiong rhizome with methanol or anhydrous ethanol, dissolving or diluting to proper concentration, high efficiency liquid chromatography detection, recording chromatogram map, and calculating relative retention time and relative peak area of the fingerprint peaks by referring to chromatograph peaks of ligustilide to establish the standard fingerprint. The present invention has the advantages of simultaneous measurement of two effective parts including water soluble and volatile oil in angelica and Chuanxiong rhizome and their compound preparation, and being scientific, simple, stable, accurate and high in repeatability.

Description

technical field [0001] The present invention relates to a research on fingerprints by high performance liquid chromatography (HPLC), to establish standard fingerprints of Chinese medicinal materials Angelica sinensis and Rhizoma Chuanxiong and preparations prepared by them alone or in combination, and to verify the authenticity of Chinese medicinal materials and preparations. and quality monitoring. Background technique [0002] Angelica sinensis is the dried root of Umbelliferae Angelica sinensis (Oliv.) Diels, and Chuanxiong is the dried rhizome of Umbelliferae Ligusticum chuanxiong Hort. The combined use of the two is called bergamot powder, which is published in "Thousand Gold Prescriptions", the name is "Yifang Leiju". They complement each other, promote blood circulation, nourish blood, and promote qi. It can control the dryness of Chuanxiong, and the dryness of Chuanxiong can control the greasyness of Angelica, dispel phlegm without hurting qi and blood, nourish bloo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06
Inventor 崔福德冯丽莉胡杰
Owner SHENYANG PHARMA UNIVERSITY
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