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Method of evaluating the transprency and moisture retention of horny layer by using oxidized protein in the horny layer as indication

A technology for stratum corneum and transparency, applied in the field of evaluation of the transparency and water retention of stratum corneum using oxidized protein in the stratum corneum as an index

Active Publication Date: 2007-03-14
SHISEIDO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although an unclear relationship between oxidized proteins and skin damage is currently predicted, it has not been clarified how the oxidation of stratum corneum proteins actually affects the properties of the skin

Method used

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  • Method of evaluating the transprency and moisture retention of horny layer by using oxidized protein in the horny layer as indication
  • Method of evaluating the transprency and moisture retention of horny layer by using oxidized protein in the horny layer as indication
  • Method of evaluating the transprency and moisture retention of horny layer by using oxidized protein in the horny layer as indication

Examples

Experimental program
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Effect test

Embodiment

[0049] Test 1: Evaluation of "water retention" of the stratum corneum using stratum corneum oxidized protein as an index (1)

[0050] Dried pigskin (Aloasque, Taiho Pharmaceutical Co., Ltd.) was immersed in water for 3 days. The pigskin was taken out, immersed in 0, 1, 10, and 100 mM acrolein (Tokyo Chemical Industry Co., Ltd.) solutions for 3 hours, and oxidized. When acrolein acts on protein, it is oxidized to form an aldehyde-protein adduct. After the oxidation treatment, wash with water for 1 hour. After washing, start drying on a petri dish with the horny layer side up (humidity 50%, temperature 25° C.), and measure for 15 minutes with Skikon 200 (Ai Bii Es Co., Ltd.) After the stratum corneum moisture content.

[0051] The results of the water content of the stratum corneum are shown in FIG. 1 as skin electrical conductivity values. As shown in FIG. 1 , a concentration-dependent decrease in water content due to oxidation treatment by acrolein was observed. That is, i...

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Abstract

It is intended to provide a method of evaluating the transparency and moisture retention of the horny layer by using oxidized protein in the horny layer as an indication.

Description

technical field [0001] The present invention relates to a method for evaluating the transparency and water retention of the stratum corneum using oxidized protein in the stratum corneum as an index; a method for detecting the degree of oxidation of proteins in the stratum corneum using the transparency and water retention of the stratum corneum as an index; It relates to a method for maintaining or improving the transparency and water retention of the stratum corneum by inhibiting the oxidation of proteins in the stratum corneum. Background technique [0002] From the viewpoint of performing proper skin care for maintaining healthier skin, it is important to accurately grasp skin texture (or skin condition). Therefore, when skin care is carried out with cosmetics, people evaluate the skin quality of the cosmetics user by, for example, asking a beauty technician or the like. In addition, in order to objectively evaluate the skin quality, various measuring instruments are use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50A61K8/00G01N33/53A61K8/02A61Q19/00G01N21/64G01N33/58G01N33/68
CPCA61Q19/00G01N2021/7786G01N21/6428G01N33/6881A61K8/02G01N33/582G01N33/50
Inventor 岩井一郎平尾哲二
Owner SHISEIDO CO LTD
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