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DNA methylation markers for metastatic prostate cancer

a prostate cancer and methylation marker technology, applied in the field of cancer, to achieve the effect of rapid and cost-effectiv

Inactive Publication Date: 2014-09-18
THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention introduces a new technology and method called MBD-SNP that enables the simultaneous analysis of genetic and epigenetic markers on a genome-scale. This approach involves using a methyl-binding domain polypeptide to enrich methylated DNA fragments from a specimen. These fragments are then hybridized to high-density oligonucleotide microarrays and compared with the total input to assess methylation, allele-specific methylation, and copy number simultaneously. This method provides a cost-effective and rapid way to analyze genome-scale markers.

Problems solved by technology

Understanding which of these alterations “drive” cancer initiation and progression to metastatis, and which of these are merely “passengers”, not involved in the chain of causation, represents a major translational challenge.

Method used

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  • DNA methylation markers for metastatic prostate cancer
  • DNA methylation markers for metastatic prostate cancer
  • DNA methylation markers for metastatic prostate cancer

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[0105]The following examples are put forth so as to provide those of ordinary skill in the art with a complete disclosure and description of how the compounds, compositions, articles, devices, and / or methods described and claimed herein are made and evaluated, and are intended to be purely illustrative and are not intended to limit the scope of what the inventors regard as their invention. Efforts have been made to ensure accuracy with respect to numbers (e.g., amounts, temperature, etc.) but some errors and deviations should be accounted for herein. Unless indicated otherwise, parts are parts by weight, temperature is in degrees Celsius or is at ambient temperature, and pressure is at or near atmospheric. There are numerous variations and combinations of reaction conditions, e.g., component concentrations, desired solvents, solvent mixtures, temperatures, pressures and other reaction ranges and conditions that can be used to optimize the product purity and yield obtained from the d...

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Abstract

The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for assessing prostate cancer. In a specific embodiment, present inventors have developed and applied a new technology and associated computation methods enabling simultaneous genome-scale analysis of genetic (copy number) and epigenetic (total methylation (TM) and allele-specific methylation (ASM) alternation, This method, called MBD-SNP, features affinity enrichment or methylated genomic DNA fragments using a methyl-binding domain polypeptide.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of U.S. Provisional Application No. 61 / 755,688, filed Jan. 23, 2013, which is incorporated herein by reference in its entirety.STATEMENT OF GOVERNMENTAL INTEREST[0002]This invention was made with government support under grant nos. CA58236, CA070196, CA113374, CA135008, GM083084, awarded by the National Institutes of Health and grant no. W81XWH-08-1-0049, awarded by the Department of Defense ARMY / MRMC. The government has certain rights in the invention.FIELD OF THE INVENTION[0003]The present invention relates to the field of cancer. More specifically, the present invention provides methods and compositions useful for assessing prostate cancer.BACKGROUND OF THE INVENTION[0004]Cancer is thought to arise from a series of somatic genome and epigenome defects that allow the cell to evade the rules that control the growth and organization of normal cells (1, 2). In order for genetic and epigenetic somatic gen...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q1/6855C12Q1/6886C12Q2600/154C12Q2537/164C12Q2565/501C12Q2565/531
Inventor YEGNASUBRAMANIAN, SRINIVASANBOVA, G. STEVENARYEE, MARTINISAACS, WILLIAM B.NELSON, WILLIAM G.
Owner THE JOHN HOPKINS UNIV SCHOOL OF MEDICINE
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