Aseptic tissue processing method, kit and device
a technology of aseptic tissue and kit, applied in the direction of biomass after-treatment, specific use bioreactor/fermenter, biochemical apparatus and processes, etc., can solve the problems of labour-intensive and labor-intensive manual or semi-automatic solid tissue processing steps, and achieve rapid pre-sterilisation environment, reduce the time required and the risk of operator exposure, the effect of eliminating the risk of contamination
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[0191]The method of the invention is exemplified according to the following process. It is clearly stated that other than the essential features of the method, the various optional steps listed herein can be independently combined to achieve the relevant technical advantages associated with the type of sampling and result to be achieved.
[0192]A semi-automatic aseptic tissue processing method comprising: automatically determining aseptic disaggregation tissue processing steps and one or more further tissue processing steps and their associated conditions from a digital tag identifier on an aseptic processing kit, optionally in accordance with the kit described herein; placing a tissue sample into a flexible plastic container of the aseptic processing kit; and processing the tissue sample by automatically executing the one or more tissue processing steps by communicating with and controlling the disaggregation module; the optional enrichment module; and the stabilisation module.
[0193]...
example 1
[0256]Impact of the Length of Disaggregation
[0257]Peripheral blood mononuclear cells were physically disaggregated for 0, 1, 5 & 10 minutes continuously before a being cultured in vitro for 0, 24 & 96 hours to assess cell recovery. The results demonstrate the physical process has negligible impact over 1 or 5 minutes and at 10 minutes the impact was transient where and initial reduction in viable cells at 0 hours was equivalent to non-disaggregated cells at 24 & 96 hours (FIG. 5).
example 2
[0258]Solid Tissue Sample Size, Volume of Digestion Media, Disaggregation and Incubation Times
[0259]Conditions of: Solid tissue size, volume of digestion media, disaggregation time and incubation conditions have been tested and demonstrate full disaggregation of solid tissue (Table 1) except where the volume of digestion media cushioned the solid tissue during the disaggregation process resulting in 30-50% of the solid tissue remaining intact.
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