High speed, high resolution compositions, methods and kits for capillary electrophoresis
a technology of capillary electrophoresis and composition, applied in the direction of liquid/fluent solid measurement, fluid pressure measurement, peptide, etc., can solve the problems of impeded capillary electrophoresis application to situations, complicated separation by capillary electrophoresis, and magnitude of eo
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example 1
Preparation of a Non-crosslinked Acrylamide Polymer by Solution Polymerization
[0059]A solution containing 94.50 g distilled water (18 M ohm-cm) and 32.02 g (0.129 mol) of a 28.57 wt % acrylamide solution (Bio-Rad, Hercules, Calif.) was prepared in a 500-mL three-necked round bottom flask fitted with a 2″ Teflon blade for mechanical stirring, a bleeding tube for purging, and a thermometer. Ultrapure helium (99.99%) was bubbled into the solution, with constant mechanical stirring, at a rate of 150 mL / min for 120 minutes, to deoxygenate the solution. To this deoxygenated solution, 1.0 mL (13.06 mmol) of 2-propanol (isopropanol) and 4.0 mL (0.35 mmol) of a 1.99 wt % ammonium persulfate (99.99% pure, Aldrich) solution were added with syringes. The flask was immersed in an oil bath at 50±1° C. for 120 minutes with constant mechanical stirring at 150 rpm and helium purging at 150 ml / minute. The reaction was quenched by the addition of 200 mL of distilled water with stirring and bubbled wit...
example 2
Preparation of Another Non-Crosslinked Acrylamide Polymer by Solution Polymerization
[0062]A second non-crosslinked acrylamide polymer was prepared as described in Example 1, except that 2.0 mL (26.12 mmol) of isopropanol were added to the deoxygenated solution. The skilled artisan will understand that isopropanol serves as a chain transfer agent, limiting the molecular weight of the polymer as it is prepared. Thus, by varying the amount of isopropanol, the molecular weight of the polymer may be altered.
[0063]The resulting water-clear solution was dialyzed and lyophilized, as in Example 1. The yield was 9.0 g of non-crosslinked arylamide polymer (98% yield). The polymer was characterized by gel permeation chromatography and was found to have a Mn of 310 kDa, a Mw of 1376 kDa (1.4 M in FIGS. 1-3), and a polydispersity of 4.40. The Mw as determined by batch mode light scattering was 975 kDa. (Prep. No. 2 in Table 1).
[0064]Following the in the solution polymerization procedure described...
example 3
Preparation of a Non-Crosslinked Acrylamide Polymer by Inverse Emulsion Polymerization
[0066]A fifth non-crosslinked acrylamide polymer was prepared by inverse emulsion polymerization (IEP) as follows. To a 1-L polypropylene beaker was added 100.05 g of Petrolum Special (bp 180-220° C., Fluka), 100.01 g of a 28.57 wt % acrylamide solution (Bio-Rad), 2.50 g of sorbitan monooleate (Fluka), and 1.00 mL of a 1.0109 wt % solution of ammonium persulfate (99.99%, Aldrich). The mixture was emulsified by stirring with a 2″ magnetic stir bar for 10 minutes at 800 rpm. The emulsion was then transferred into a 1-L three-necked round bottom flask equipped with a 2″ Teflon stirring blade for mechanical stirring, a bleeding tube for purging, and a thermometer. The emulsion was purged with ultra pure helium (99.99%) at a rate of 150 mL / min for 120 minutes with constant mechanical stirring at 300 rpm. To the emulsion was added 0.010 mL of N,N,N,N-tetramethylethylenediamine (ultra pure, Armesco) using...
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