Biogas residue decomposing agent with biogas output synergistic effect and application thereof

Abstract

The invention provides a biogas residue decomposing agent with a biogas output synergistic effect and an application thereof and belongs to the technical field of biotechnology. In the biogas residuedecomposing agent with the biogas output synergistic effect, cellulase and xylanase are capable of enzymatically decomposing the undigested feed residue in the biogas residue, decomposing the crude fiber plant long-chain fibers in the residue into the short-chain fibers easily absorbed and utilized by the microorganism and enzymatically decomposing the plant cell walls in the residue; the releasedxylan and non-starch polysaccharides are hydrolyzed and converted into the monosaccharides, such as glucose, which can be easily absorbed by the microorganism; the monosaccharides, such as the short-chain fibers and glucose, formed in the enzymatic decomposing process can be digested and decomposed under the effects of bacillus subtilis and bacillus licheniformis and the residue and the biogas residue can be further decomposed, so that the effect of quickly decomposing the biogas residue can be achieved.

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a biogas residue decomposer and its application. Background technique

[0002] Facing the current situation of energy reduction, biogas fermentation is an important method to develop biomass energy. Biogas fermentation is a method with high efficiency and mature technology. It will be of great significance to change the energy structure and reduce dependence on fossil fuels. For example, at Shanghai Xinghuo Farm in Taicang, Jiangsu, the manure of 2,700 dairy cows was fermented with biogas to produce nearly 3,000 m3 3 Biogas can solve the cooking gas of 2,700 households. At the same time, biogas fermentation is also an important treatment method for high-concentration organic wastewater and waste. Biogas fermentation can treat poultry and livestock manure, reduce the accumulation of sewage, and play an important role in environmental purification.

[0003] Raw materials ...

Examples

preparation example Construction

[0026] In the present invention, the preparation method of the biogas residue decomposing agent with synergistic effect on biogas production preferably includes the following steps:

[0027] 1) mixing cellulase and xylanase to obtain a compound enzyme;

[0028] 2) mixing the compounded enzyme in the step 1) with Bacillus subtilis and Bacillus licheniformis to obtain an enzyme-bacteria complex;

[0029] 3) Mixing the enzyme-bacteria complex in the step 2) with the carrier to obtain a biogas residue decomposing agent with synergistic effects on biogas production.

[0030] In the present invention, the mixing method is not particularly limited, and a mixing scheme well known to those skilled in the art can be used.

[0031] The invention provides the application of the biogas residue decomposer with biogas production synergistic effect in improving biogas production and / or biogas residue degradation efficiency in biogas fermentation.

[0032]In the present invention, the raw ma...

Embodiment 1

[0036] Bacillus licheniformis powder and Bacillus subtilis powder purchased from Shandong Gaolong Biotechnology Co., Ltd. were mixed with β-1,4-glucan-4-glucan hydrolase, β-1,4-endolog Mix glycanase and β-xylosidase, then add defatted rice bran and mix thoroughly, so that the content of each component is 2.0 for the activity of β-1,4-glucan-4-glucanohydrolase per gram of defatted rice bran ×10 5 U, β-1,4-endoxylanase, β-xylosidase is 1.0×10 5 U, the number of viable bacteria of Bacillus subtilis is 20 billion CFU, and the number of viable bacteria of Bacillus licheniformis is 10 billion CFU, and a biogas residue decomposing agent with synergistic effect on biogas production is obtained.

Embodiment 2

[0038] The original powder of Bacillus licheniformis and Bacillus subtilis purchased from Shandong Gaolong Biotechnology Co., Ltd. were mixed with β-1,4-glucan-4-glucanohydrolase and α-L-arabinosidase, Then add defatted rice bran and mix thoroughly so that the content of each component is β-1,4-glucan-4-glucan hydrolase activity per gram of defatted rice bran is 1.8×10 5 U, α-L-arabinosidase activity is 0.9×10 5 U, the number of viable bacteria of Bacillus subtilis is 18 billion CFU, and the number of viable bacteria of Bacillus licheniformis is 9 billion CFU, and a biogas residue decomposing agent with synergistic effect on biogas production is obtained.

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a biogas residue decomposer and its application. Background technique

[0002] Facing the current situation of energy reduction, biogas fermentation is an important method to develop biomass energy. Biogas fermentation is a method with high efficiency and mature technology. It will be of great significance to change the energy structure and reduce dependence on fossil fuels. For example, at Shanghai Xinghuo Farm in Taicang, Jiangsu, the manure of 2,700 dairy cows was fermented with biogas to produce nearly 3,000 m3 3 Biogas can solve the cooking gas of 2,700 households. At the same time, biogas fermentation is also an important treatment method for high-concentration organic wastewater and waste. Biogas fermentation can treat poultry and livestock manure, reduce the accumulation of sewage, and play an important role in environmental purification.

[0003] Raw materials ...

Examples

preparation example Construction

[0026] In the present invention, the preparation method of the biogas residue decomposing agent with synergistic effect on biogas production preferably includes the following steps:

[0027] 1) mixing cellulase and xylanase to obtain a compound enzyme;

[0028] 2) mixing the compounded enzyme in the step 1) with Bacillus subtilis and Bacillus licheniformis to obtain an enzyme-bacteria complex;

[0029] 3) Mixing the enzyme-bacteria complex in the step 2) with the carrier to obtain a biogas residue decomposing agent with synergistic effects on biogas production.

[0030] In the present invention, the mixing method is not particularly limited, and a mixing scheme well known to those skilled in the art can be used.

[0031] The invention provides the application of the biogas residue decomposer with biogas production synergistic effect in improving biogas production and / or biogas residue degradation efficiency in biogas fermentation.

[0032]In the present invention, the raw ma...

Embodiment 1

[0036] Bacillus licheniformis powder and Bacillus subtilis powder purchased from Shandong Gaolong Biotechnology Co., Ltd. were mixed with β-1,4-glucan-4-glucan hydrolase, β-1,4-endolog Mix glycanase and β-xylosidase, then add defatted rice bran and mix thoroughly, so that the content of each component is 2.0 for the activity of β-1,4-glucan-4-glucanohydrolase per gram of defatted rice bran ×10 5 U, β-1,4-endoxylanase, β-xylosidase is 1.0×10 5 U, the number of viable bacteria of Bacillus subtilis is 20 billion CFU, and the number of viable bacteria of Bacillus licheniformis is 10 billion CFU, and a biogas residue decomposing agent with synergistic effect on biogas production is obtained.

Embodiment 2

[0038] The original powder of Bacillus licheniformis and Bacillus subtilis purchased from Shandong Gaolong Biotechnology Co., Ltd. were mixed with β-1,4-glucan-4-glucanohydrolase and α-L-arabinosidase, Then add defatted rice bran and mix thoroughly so that the content of each component is β-1,4-glucan-4-glucan hydrolase activity per gram of defatted rice bran is 1.8×10 5 U, α-L-arabinosidase activity is 0.9×10 5 U, the number of viable bacteria of Bacillus subtilis is 18 billion CFU, and the number of viable bacteria of Bacillus licheniformis is 9 billion CFU, and a biogas residue decomposing agent with synergistic effect on biogas production is obtained.

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