Preparation method of COL/PEG@CaP biomineralization multilayer film

Abstract

The invention discloses a preparation method of a COL / PEG@CaP biomineralization multilayer film. According to the preparation method, titanium alloy is taken as a substrate, the surface adhesiveness of the titanium alloy is improved with PDA; on the PDA modified titanium alloy substrate, COL and PEG are taken as polyelectrolytes, layer-by-layer self-assembly technology is adopted to prepare a COL / PEG self-assembled multilayer film; and at last, the COL / PEG@CaP biomineralization multilayer film is prepared through biomineralization. The COL / PEG@CaP biomineralization multilayer film prepared bythe invention can effectively improve the biotoxicity of inorganic particles in vivo; the preparation process is simple, low in cost, short in period and suitable for mass production, and has a wide application prospect; the prepared (COL / PEG)@CaP mineralized film has the functions of drug loading and release, osteoblast directional differentiation control and cell differentiation degree detection, and has a wide application prospect in the field of tissue engineering.

Description

technical field

[0001] The invention belongs to the technical field of chemical composite materials, layer-by-layer self-assembly and biomineralization preparation, and relates to a method for preparing layer-by-layer self-assembled films and biomineralization ions, in particular to a COL / PEG@CaP biomineralization multilayer film Preparation. Background technique

[0002] Supramolecular chemistry is developing rapidly and attracting more and more attention from the scientific community. The goal of supramolecular chemistry is to synthesize materials in a non-covalent manner. Non-covalent forces exist everywhere in natural living systems, such as the quaternary structure of proteins and double-stranded DNA in phospholipid bilayer membranes. In particular, supramolecular nanotechnology has shown great potential in the diagnosis and treatment of diseases. Specifically, nanoparticles with tunable and diverse properties can be prepared by supramolecular self-assembly technology...

Examples

Embodiment 1

[0052] The embodiment of the present invention provides a method for preparing a COL / PEG@CaP biomineralized multilayer film, comprising the following steps:

[0053] (1) Using medical titanium alloy as the substrate, the titanium alloy substrate is subjected to plasma treatment to make its surface hydroxylated, so as to achieve better hydrophilicity, so as to facilitate the subsequent assembly process;

[0054] (2) Prepare 1 mg / mL DA solution and adjust its pH value to 8.5 with Tris solution, and magnetically stir to make DA polymerize to form a PDA solution.

[0055] (3) Soak the titanium alloy substrate in the PDA solution for 30 minutes to improve the adhesion of the surface of the substrate and make the substrate for subsequent polyelectrolyte assembly

[0056] (4) Prepare 1mg / mL COL solution, 1mg / mL PEG solution, 500mM CaCl respectively 2 solution and 300mM K 2 HPO 4 solution, and the pH value of the COL solution was adjusted to 3.5 with sodium hydroxide;

[0057] (5)...

Embodiment 2

[0067] On the basis of Example 1, the difference between this Example and Example 1 is that in Step 7), Step 5) and Step 6) are alternately repeated 10 times; other parts are completely consistent with Example 1.

Embodiment 3

[0069] On the basis of Example 1, the difference from Example 1 in this example is that in step 4), the concentrations of the configured COL solution and PEG solution are both 0.5 mg / mL, and the pH of the COL solution is adjusted to 0.5 mg / mL with sodium hydroxide. The value is adjusted to 3.5; other parts are completely consistent with Example 1.

technical field

[0001] The invention belongs to the technical field of chemical composite materials, layer-by-layer self-assembly and biomineralization preparation, and relates to a method for preparing layer-by-layer self-assembled films and biomineralization ions, in particular to a COL / PEG@CaP biomineralization multilayer film Preparation. Background technique

[0002] Supramolecular chemistry is developing rapidly and attracting more and more attention from the scientific community. The goal of supramolecular chemistry is to synthesize materials in a non-covalent manner. Non-covalent forces exist everywhere in natural living systems, such as the quaternary structure of proteins and double-stranded DNA in phospholipid bilayer membranes. In particular, supramolecular nanotechnology has shown great potential in the diagnosis and treatment of diseases. Specifically, nanoparticles with tunable and diverse properties can be prepared by supramolecular self-assembly technology...

Examples

Embodiment 1

[0052] The embodiment of the present invention provides a method for preparing a COL / PEG@CaP biomineralized multilayer film, comprising the following steps:

[0053] (1) Using medical titanium alloy as the substrate, the titanium alloy substrate is subjected to plasma treatment to make its surface hydroxylated, so as to achieve better hydrophilicity, so as to facilitate the subsequent assembly process;

[0054] (2) Prepare 1 mg / mL DA solution and adjust its pH value to 8.5 with Tris solution, and magnetically stir to make DA polymerize to form a PDA solution.

[0055] (3) Soak the titanium alloy substrate in the PDA solution for 30 minutes to improve the adhesion of the surface of the substrate and make the substrate for subsequent polyelectrolyte assembly

[0056] (4) Prepare 1mg / mL COL solution, 1mg / mL PEG solution, 500mM CaCl respectively 2 solution and 300mM K 2 HPO 4 solution, and the pH value of the COL solution was adjusted to 3.5 with sodium hydroxide;

[0057] (5)...

Embodiment 2

[0067] On the basis of Example 1, the difference between this Example and Example 1 is that in Step 7), Step 5) and Step 6) are alternately repeated 10 times; other parts are completely consistent with Example 1.

Embodiment 3

[0069] On the basis of Example 1, the difference from Example 1 in this example is that in step 4), the concentrations of the configured COL solution and PEG solution are both 0.5 mg / mL, and the pH of the COL solution is adjusted to 0.5 mg / mL with sodium hydroxide. The value is adjusted to 3.5; other parts are completely consistent with Example 1.

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