The invention discloses a separation and purification process of oleoyl monoethanolamine, which comprises the following steps: (1) dispersing an oleoyl monoethanolamine crude product a obtained by reacting
ethanolamine with a
methyl oleate crude product in low-carbon
alcohol, heating, stirring and dissolving, and filtering to remove insoluble substances to obtain filtrate; (2) adding deionized water into the obtained filtrate, cooling, standing for
crystallization, filtering, and removing a
filter cake to obtain filtrate; (3) supplementing
ethanolamine into the filtrate, freezing, standing for
crystallization, filtering and
drying to obtain an oleoyl monoethanolamine
solid crystal crude product b; (4) dissolving the crude product b in a low-carbon
alcohol aqueous solution, adding
organic acid to adjust the pH value of the
system, standing at low temperature for
crystallization, filtering and
drying to obtain a flaky oleoyl monoethanolamine
crystal product with squamous gloss; the whole process is easy to industrialize, the content of the prepared oleoyl monoethanolamine is 90% or above, the purity is high, and the method has important significance in the oil industry,
health food industry and
medicine industry in China.