The invention discloses a skin care product for promoting RNA transcription. The skin care product comprises the following components in parts by mass: 2.3 to 5.1 parts of triglyceride decanoate, 4 to6 parts of squalane, 2 to 3.6 parts of ethylhexyl palmitate, 5 to 7.3 parts of isopropyl myristate, 3.2 to 4.4 parts of mink oil, 1.3 to 3.3 parts of PEG-7 glyceryl cocoate, 20 to 40.8 parts of deionized water, 5 to 7 parts of seaweed oligosaccharin, 7 to 10 parts of glycerol, 2.3 to 3.6 parts of microorganism C, 5.5 to 8.6 parts of retinol, 3.8 to 6.5 parts of peptide, 3.3 to 4.5 parts of coenzyme Q10, 20 to 30 parts of recombinant human III type human-like collagen and 1.5 to 3.4 parts of interleukin 13. The invention further provides a preparation process of the skin care product for promoting RNA transcription. A preparation method comprises the following steps of S1, selecting 2.3 to 5.1 parts of triglyceride decanoate, 4 to 6 parts of squalane, 2 to 3.6 parts of ethylhexyl palmitate, 5 to 7.3 parts of isopropyl myristate, 3.2 to 4.4 parts of mink oil and 1.3 to 3.3 parts of PEG-7 glyceryl cocoate, and starting steam for heating; S2; S3; S4; and S5. The skin care product is reasonable in structure; transcription of type III collagen genes can be accelerated; the higher the transcription number is, the more generated messenger RNA is; and the content of skin collagen is greatly increased accordingly.