1 results about "Ionic calcium" patented technology

Provided are a hydrogel microsphere for constructing a bone organoid, a kit, a bone organoid, a bone organoid-loaded hydrogel, and a preparation method therefor and the use thereof. The kit comprises (1) a gel system and / or (2) a culture medium system, and further comprises (3) a phosphorus source and / or a calcium source, wherein an addition amount of the phosphorus source in the gel system or culture medium system is 24000-33000 mg / L; and an addition amount of the calcium source in the culture medium system is 2-8 mmol / L. The calcium source and the phosphorus source required for mineralization are introduced into a hydrogel and a culture matrix during the in vitro culture of bone organoids. Without external assistance, only a single cell type is used, along with simple ionic calcium and phosphorus, and microspheres are used to regulate the spatial distribution of calcium and phosphorus, which is beneficial to promoting cellular mineralization and osteogenesis. The in-situ mineralization method can effectively guide osteogenesis, mineralization, cell layer formation, shaping and remodeling, promote the development of bone organoids, effectively shorten the bone repair cycle, and achieve greater safety and broader applicability.