The application provides a
HPLC fingerprint spectrum and multi-index component
content determination method of Qiling Fuzheng Qingjie granules. The method optimizes the preparation process of the
test sample through
single factor test, systematically screens the chromatographic conditions, establishes the
HPLC fingerprint spectrum of 15 batches of samples, calibrates 43 common peaks and completes the attribution identification of medicinal materials; meanwhile, the method establishes a synchronous
content determination method of 17 kinds of components including flavonoids, phenolic acids, triterpenes and other kinds of components, and through complete methodology
verification, all indexes meet the requirements of the quality analysis methodology of traditional Chinese medicines, so that the quality of Qiling Fuzheng Qingjie granules can be comprehensively and effectively controlled. The application has the characteristics of high analysis efficiency, simple and easy method, strong specificity and the like, and can provide
technical support for the
quality control of Qiling Fuzheng Qingjie granules.