This invention discloses a pulse output circuit and a high-voltagepulse power supply. The pulse output circuit includes: at least one output unit; each output unit includes two basic units, each basic unit including a power supply unit and a pulse network; each pulse network includes a bridge arm and an energy storagecapacitor, the high-potential ends of which are connected to the positive output terminal of the corresponding power supply unit, and the low-potential ends of which are connected to the negative output terminal of the corresponding power supply unit. The midpoint of the first pulse network bridge arm is the positive output terminal of the corresponding output unit, the low-potential end of the first pulse network bridge arm is connected to the high-potential end of the second pulse network bridge arm, and the midpoint of the second pulse network bridge arm is the output terminal of the corresponding output unit; the positive output terminal of the first output unit is the positive output terminal of the pulse output circuit, the negative output terminal of the preceding output unit is connected to the positive output terminal of the following output unit, and the negative output terminal of the last output unit is the negative output terminal of the pulse output circuit. This invention can improve the accuracy of the pulse output voltage and eliminate pulse tailing.