Human enterovirus(EV) fluorescence quantitative PCR detecting technology

Abstract

This invention relates to one human intestinal canal virus fluorescence meter test technique in the field of virus nuclear test technique, which adopts common upstream object, common downstream object and common fluorescence detector for EV each hypotype, wherein, the detector 5'end is labeled with fluorescence emission base group 6- carboxyl fluorandiol and 3'labeled fluorescence quencher base group and 6-carboxyl tetramethyl rhodamine. Due to the test agent case, it tests each EV hypotype standard blood serum for online test and to get each parameter as the following: abnormal property for 100 percent; sensitivity for 95 percent, repetitiveness CV less than 10 percent and minimum content of EV as 500 copy each ml.

Description

technical field

[0001] The invention belongs to the technical field of viral nucleic acid detection, in particular to a human enterovirus (EV) fluorescent quantitative PCR detection technology, which is suitable for quantitative detection of human enterovirus, diagnosis of human enterovirus infectious diseases, and epidemiological investigation , and to evaluate the effectiveness of prevention and treatment. Background technique

[0002] Human enteroviruses (enteroviruses, EVs) belong to the Picornaviridae family, including polioviruses (serotypes 1-3), coxsackieviruses A (serotypes 1-22, 24) and coxsackieviruses B (serotypes 1-6), echoviruses (serotypes 1-9, 11-27, 29-31) and new enteroviruses (serotypes 68-71).

[0003] The most common symptom of enteroviral infection is nonspecific fever, sometimes accompanied by rash. The acute viral syndromes caused mainly include encephalitis, myocarditis (especially caused by Coxsackie B group virus), hemorrhagic conjunctivitis (esp...