Natural antibiotic composition extracted from rust-colored crotalaria herb and its extraction process

Abstract

The present invention discloses one kind of natural antibiotic composition extracted from rust-colored crotalaria herb and its extraction process. The extraction process includes crushing dry rust-colored crotalaria herb and 40-200 mesh sieving, soaking in solvent for 1-5 times of 12-24 hr each, vibrating in shaking table of rotation speed 180-250 rpm at 30-40 deg.c for 12-24 hr, suction filtering to obtain extracted liquid, and drying to recover solvent and obtain the composition. The solvent is water, 95 % concentration alcohol solution or ethyl acetate. The natural antibiotic composition has excellent bactriostasis on staphylococcus aureus, colibacillus, fecal streptococcus, etc.

Description

technical field

[0001] The invention relates to an antibacterial composition, in particular to a natural antibacterial composition extracted from bellflowers, and also to an extraction method thereof. Background technique

[0002] Antibacterial drugs are the most widely used drugs in clinical practice, but there are many problems in the application of these drugs, such as drug resistance, toxic and side effects, bacterial flora caused by antibacterial drugs, superinfection, nosocomial infections, and allergies etc., especially the rapid rise of bacterial resistance. There is no effective drug available for the serious infection caused by this type of bacteria, which poses a great threat to human health. Therefore, at present, people develop new antibacterial drugs from natural resources as a focus of antibacterial drug research.

[0003] Bell grass is the whole grass or the whole grass with roots of the leguminous plant Crotalaria ferruginea Grah. Bellflower is a perennia...

Examples

Embodiment 1

[0014] Crush the dried bellflower through a 40-mesh sieve, weigh 150g, add 1000ml of water, soak for 2 times, each time for 12h, shake on a constant temperature shaker with a temperature of 37°C and a speed of 200r / min for 12h, pump The extract was obtained by filtration, frozen at -80°C for 12 hours, and then dried with a vacuum freeze dryer for 24 hours to obtain a dried product.

[0015] Take 0.3g of the dry product of the bellflower water extract, dissolve it in 3mL DMF respectively as the stock solution, pass through a bacterial filter, and dilute it with LB liquid medium to 1:2, 1:4, 1:8, 1 : 16, 1: 32, 1: 64, 1: 128, 1: 256, each with 8 concentrations, that is, the concentration of stock solution is 50, 25, 2.5, 6.25, 3.1, 1.5, 0.75, 0.375mg / mL, each tube 300ul. Three parallel tubes were set up for each concentration, and LB liquid culture medium without extract was set up as a control tube. Then, 30 uL of the pre-prepared bacterial suspension (OD value at 600 nm is a...

Embodiment 2

[0019] Crush the dried bellflower through a 120-mesh sieve, weigh 150g, add 1000ml, 95% alcohol, soak for 3 times, each time for 18h, shake on a constant temperature shaker with a temperature of 30°C and a speed of 250r / min After 18 hours, the extract was obtained by suction filtration, concentrated under reduced pressure at 40°C to remove alcohol, frozen at -80°C for 12 hours, and dried with a vacuum freeze dryer for 24 hours to obtain a dry product.

[0020] Take 0.3g of the dry extract of Bellflower 95% alcohol, dissolve it in 3mL DMF as the stock solution, pass it through a bacterial filter, and dilute it with LB liquid medium to 1:2, 1:4, 1:8 respectively , 1:16, 1:32, 1:64, 1:128, 1:256 each with 8 concentrations, that is, the concentration of stock solution is 50, 25, 12.5, 6.25, 3.1, 1.5, 0.75, 0.375mg / mL, 300ul per tube. Three parallel tubes were set up for each concentration, and LB liquid culture medium without extract was set up as a control tube. Then, 30 uL of ...

Embodiment 3

[0023] Crush the dried bellflower through a 200-mesh sieve, weigh 150g, add 1000ml of ethyl acetate, soak for 5 times, each time for 24h, place it on a constant temperature shaker at a temperature of 40°C and a rotation speed of 180r / min for 24h , Suction filtration to obtain the extract, concentrated under reduced pressure at 45°C to remove ethyl acetate to obtain a dry product.

[0024] Take 0.3g of the dry product of the ethyl acetate extract of Bellflower, dissolve it in 3mL DMF as the stock solution, pass it through a bacterial filter, and dilute it with LB liquid medium to 1:2, 1:4, 1:8 respectively , 1:16, 1:32, 1:64, 1:128, 1:256 each with 8 concentrations, that is, the concentration of stock solution is 50, 25, 2.5, 6.25, 3.1, 1.5, 0.75, 0.375mg / mL, 300ul per tube. Three parallel tubes were set up for each concentration, and LB liquid culture medium without extract was set up as a control tube. Then, 30 uL of the pre-prepared bacterial suspension (OD value at 600 nm...

technical field

[0001] The invention relates to an antibacterial composition, in particular to a natural antibacterial composition extracted from bellflowers, and also to an extraction method thereof. Background technique

[0002] Antibacterial drugs are the most widely used drugs in clinical practice, but there are many problems in the application of these drugs, such as drug resistance, toxic and side effects, bacterial flora caused by antibacterial drugs, superinfection, nosocomial infections, and allergies etc., especially the rapid rise of bacterial resistance. There is no effective drug available for the serious infection caused by this type of bacteria, which poses a great threat to human health. Therefore, at present, people develop new antibacterial drugs from natural resources as a focus of antibacterial drug research.

[0003] Bell grass is the whole grass or the whole grass with roots of the leguminous plant Crotalaria ferruginea Grah. Bellflower is a perennia...

Examples

Embodiment 1

[0014] Crush the dried bellflower through a 40-mesh sieve, weigh 150g, add 1000ml of water, soak for 2 times, each time for 12h, shake on a constant temperature shaker with a temperature of 37°C and a speed of 200r / min for 12h, pump The extract was obtained by filtration, frozen at -80°C for 12 hours, and then dried with a vacuum freeze dryer for 24 hours to obtain a dried product.

[0015] Take 0.3g of the dry product of the bellflower water extract, dissolve it in 3mL DMF respectively as the stock solution, pass through a bacterial filter, and dilute it with LB liquid medium to 1:2, 1:4, 1:8, 1 : 16, 1: 32, 1: 64, 1: 128, 1: 256, each with 8 concentrations, that is, the concentration of stock solution is 50, 25, 2.5, 6.25, 3.1, 1.5, 0.75, 0.375mg / mL, each tube 300ul. Three parallel tubes were set up for each concentration, and LB liquid culture medium without extract was set up as a control tube. Then, 30 uL of the pre-prepared bacterial suspension (OD value at 600 nm is a...

Embodiment 2

[0019] Crush the dried bellflower through a 120-mesh sieve, weigh 150g, add 1000ml, 95% alcohol, soak for 3 times, each time for 18h, shake on a constant temperature shaker with a temperature of 30°C and a speed of 250r / min After 18 hours, the extract was obtained by suction filtration, concentrated under reduced pressure at 40°C to remove alcohol, frozen at -80°C for 12 hours, and dried with a vacuum freeze dryer for 24 hours to obtain a dry product.

[0020] Take 0.3g of the dry extract of Bellflower 95% alcohol, dissolve it in 3mL DMF as the stock solution, pass it through a bacterial filter, and dilute it with LB liquid medium to 1:2, 1:4, 1:8 respectively , 1:16, 1:32, 1:64, 1:128, 1:256 each with 8 concentrations, that is, the concentration of stock solution is 50, 25, 12.5, 6.25, 3.1, 1.5, 0.75, 0.375mg / mL, 300ul per tube. Three parallel tubes were set up for each concentration, and LB liquid culture medium without extract was set up as a control tube. Then, 30 uL of ...

Embodiment 3

[0023] Crush the dried bellflower through a 200-mesh sieve, weigh 150g, add 1000ml of ethyl acetate, soak for 5 times, each time for 24h, place it on a constant temperature shaker at a temperature of 40°C and a rotation speed of 180r / min for 24h , Suction filtration to obtain the extract, concentrated under reduced pressure at 45°C to remove ethyl acetate to obtain a dry product.

[0024] Take 0.3g of the dry product of the ethyl acetate extract of Bellflower, dissolve it in 3mL DMF as the stock solution, pass it through a bacterial filter, and dilute it with LB liquid medium to 1:2, 1:4, 1:8 respectively , 1:16, 1:32, 1:64, 1:128, 1:256 each with 8 concentrations, that is, the concentration of stock solution is 50, 25, 2.5, 6.25, 3.1, 1.5, 0.75, 0.375mg / mL, 300ul per tube. Three parallel tubes were set up for each concentration, and LB liquid culture medium without extract was set up as a control tube. Then, 30 uL of the pre-prepared bacterial suspension (OD value at 600 nm...