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Method for producing non-potassium cyanide haeomolytic agent

A potassium cyanide hemolytic agent and a production method technology, which are applied in the directions of biochemical equipment and methods, biological testing, and microbial determination/inspection, can solve problems such as inconvenience, heavy economic burden on hospitals and patients, and increase production costs, and achieve Simple production process, easy to achieve the effect of industrialization

Inactive Publication Date: 2007-08-22
NANCHANG UNIV
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AI Technical Summary

Problems solved by technology

[0002] The three-group blood cell analyzer is a blood routine testing instrument widely used in my country at present. For a long time, such instruments and reagents have been imported in my country. Due to the high price of reagents and untimely supply, it has brought heavy economic burdens to hospitals and patients. and inconvenience
[0005] The main disadvantages of the existing TEK blood cell analyzer hemolytic agent are: 1. High product cost; 2. Use highly toxic potassium cyanide to measure hemoglobin; 3. Adding preservatives affects the classification and measurement results and increases production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1. Taking the domestic TEK-II three-group hematology analyzer hemolytic agent as an example, the process steps are:

[0020] 1. Preparation of purified water: Purified water is prepared from tap water. The steps include (1) electrodialysis desalination; (2) anion and cation exchange column desalination; Less than 5μs / cm;

[0021] 2. Buffer preparation: prepare 1 / 30M, PH=7.0 Na 2 HPO 4 -KH 2 PO 4 100 liters of buffer solution, stir well, filter with 0.22 micron ultrafiltration;

[0022] 3. Dissolving ethylenediaminetetraacetic acid: dissolve 100 grams of ethylenediaminetetraacetic acid with 10-20 liters of Na 2 HPO 4 -KH 2 PO 4 Heat the buffer solution to dissolve, stir well, and cool down for later use;

[0023] 4. Dissolving dodecyl quaternary ammonium salt: 2000 grams of dodecyl quaternary ammonium salt with 10-20 liters of Na 2 HPO 4 -KH 2 PO 4 Heat the buffer to dissolve, stir well, and cool down for later use;

[0024] 5. Preparation at con...

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PUM

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Abstract

A produce method of hemolysis agent used by home TEK style three grouping blood corpuscle analyzer with out potassium cyanide. Using dodecyl season amine salt as the main function reagent of the hemolysis agent, using innoxious material instead of potassium cyanide to measure hematoglobin, its technology steps are: (1) prepare purify water, (2)confect buffer, (3) dissolve EDTA, (4) dissolve dodecyl season amine salt, (5) constant volume, (6) filtration and filling. The technology recipe of the production is reasonable, the raw material is easy to get, and its process is easy, the cost is low, and it did not add antiseptic.

Description

technical field [0001] The invention relates to a production method of a potassium cyanide-free hemolytic agent, in particular to a production method of a non-toxic hemolytic agent used in a domestic TEK series three-group blood cell analyzer without potassium cyanide. Background technique [0002] The three-group blood cell analyzer is a blood routine testing instrument widely used in my country at present. For a long time, such instruments and reagents have been imported in my country. Due to the high price of reagents and untimely supply, it has brought heavy economic burdens to hospitals and patients. and inconvenience. Among the triplicate blood cell analyzers that are widely used in my country, there are no less than 20 brands of various brands. Due to the differences in the dilution ratios and measurement methods of various instruments, the reagent formulas of various instruments are different. Different models and manufacturers The hematology analyzer must use special...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48C12Q1/02
Inventor 罗舜菁刘成梅邹常春涂宗财
Owner NANCHANG UNIV
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