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Method of making excised aleuriospore permanent slide exemplar

A technology for making powdery spores and specimens, which is applied in the preparation of test samples, instruments, teaching models, etc., can solve the problems of short storage and use period, input manpower, material resources and time, and achieve the effect of saving manpower

Inactive Publication Date: 2007-10-31
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, for a long time, isolated powdery spores, such as: smut, powdery powder, etc., can only be made into semi-permanent slide specimens. Due to the short storage period, it is necessary to continuously invest manpower, material resources and time Making such pathogen slides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The present invention mainly adopts following method steps:

[0017] (1) Use a straw to add 50% alcohol into the centrifuge tube. The amount added is based on the amount that can bury the spores. Use a picking needle to pick the isolated powdery spores into the alcohol in the centrifuge tube, stir fully, and place for 6 hours. ;

[0018] (2), put the centrifuge tube into the centrifuge, centrifuge for 3 minutes at 500 rpm;

[0019] (3) Use a straw to absorb the alcohol in the centrifuge tube, add 50% alcohol, and place it for 6 hours;

[0020] (4), use a straw to absorb the alcohol in the centrifuge tube, add xylene, and place it for 6 hours;

[0021] (5), by weight, the mixed solution of 30 parts of xylene and 70 parts of resin glue is added in the centrifuge tube, fully stirs, makes it to become spore suspension;

[0022] (6) Use a glass rod to dip a drop of spore suspension onto the glass slide, and then cover it with a cover glass to make a permanent glass slide ...

Embodiment 2

[0025] The present invention mainly adopts following method steps:

[0026] (1) Use a straw to add alcohol with a concentration of 60% to the centrifuge tube. The amount added is subject to the ability to bury the spores. Use a picking needle to pick the isolated powdery spores into the alcohol in the centrifuge tube, stir fully, and place it for 10 hours. ;

[0027] (2), put the centrifuge tube into the centrifuge, 800 rpm, and centrifuge for 10 minutes;

[0028] (3), absorb the alcohol in the centrifuge tube with a straw, add 60% alcohol, and place it for 10 hours;

[0029] (4), absorb the alcohol in the centrifuge tube with a straw, add xylene, and place it for 10 hours;

[0030] (5), by weight, the mixed solution of 40 parts of xylene and 60 parts of resin glue is added in the centrifuge tube, fully stir, make it become spore suspension;

[0031] (6) Use a glass rod to dip a drop of spore suspension onto the glass slide, and then cover it with a cover glass to make a pe...

Embodiment 3

[0034] The present invention mainly adopts following method steps:

[0035] (1) Use a straw to add alcohol with a concentration of 70% to the centrifuge tube. The amount added is subject to the ability to bury the spores. Use a picking needle to pick the isolated powdery spores into the alcohol in the centrifuge tube, stir fully, and place it for 12 hours. ;

[0036] (2), put the centrifuge tube into the centrifuge, 1200 rpm, and centrifuge for 15 minutes;

[0037] (3) Use a straw to absorb the alcohol in the centrifuge tube, add 75% alcohol, and place it for 12 hours;

[0038] (4), absorb the alcohol in the centrifuge tube with a straw, add xylene, and place it for 12 hours;

[0039] (5), by weight, the mixed solution of 50 parts of xylene and 50 parts of resin glue is added in the centrifuge tube, fully stir, make it become spore suspension;

[0040] (6) Use a glass rod to dip a drop of spore suspension onto the glass slide, and then cover it with a cover glass to make a ...

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PUM

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Abstract

This invention discloses one ion powder bubble permanent specimen process method, which comprises the following steps: a, adding the absorptive alcohol into centrifuge tube and picking up the bubbles into the tubes; b, putting into centrifuge machine; c, absorbing the alcohol in the tube and adding some; d, using absorptive tube for alcohol in tube and adding dimethylbenzene; e, adding the mixture of dimethylbenzene and resin glue into the tube; f, using glass bar to dip one drop of bubble floating on the load slice then covering to process permanent glass specimen.

Description

technical field [0001] The invention relates to a method for preparing a specimen, in particular to a method for preparing a permanent glass slide specimen of isolated powdery spores. Background technique [0002] The slide specimen of plant pathogenic bacteria is an important experimental material in the experimental teaching of plant pathology. Plant pathogen slide specimens are divided into permanent slide specimens and semi-permanent slide specimens. Permanent slide specimens can be stored permanently and used permanently. The semi-permanent slide specimens have a short shelf life and can be used for about 3 years. Regarding the method of making permanent slide specimens, in monographs (plant disease research methods) and textbooks (experimental instructions), only the pathogenic bacteria structure together with the host tissue is introduced, such as: ascus with host tissue, conidia How to make permanent glass slide specimens for isolated powdery spores has no introdu...

Claims

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Application Information

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IPC IPC(8): G01N1/28G09B23/38
Inventor 刘建华
Owner HENAN AGRICULTURAL UNIVERSITY
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