Chinese medicine composition for treating endotoxemia and its prepn process

Abstract

The present invention relates to one kind of Chinese medicine composition for treating endotoxemia and its preparation process. The Chinese medicine composition consists of effective components prepared with capillary artemisia, cape jasmine, rhubarb, red sage, honeysuckle and other five kinds of Chinese medicinal materials in certain weight proportion, and pharmaceutically acceptable carrier. The Chinese medicine composition is used in treating endotoxemia and various diseases with endotoxemia complication, and has especially high curative effect on endotoxin induced endotoxin shock and multiple organ incompetence.

Description

Technical field

[0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a compound pharmaceutical preparation of an anti-endotoxemia traditional Chinese medicine composition. Background technique

[0002] Endotoxins are lipopolysaccharides that make up the cell membranes of Gram-negative bacteria. It has a double impact on the body: a small amount of endotoxin stimulation can enhance the body's specific and non-specific immunity, induce interferon, etc., while a large amount of endotoxin entering the circulatory system will cause endotoxemia, which can cause a wide range of pathological reactions. Such as multiple organ dysfunction syndrome, disseminated intravascular coagulation, multiple organ failure and shock. Septic shock and subsequent multiple organ dysfunction syndrome are the main causes of death in patients with sepsis. As a means for preventing and treating endotoxemia, antibiotics have conventionally been...

Examples

Embodiment 1

[0022] Embodiment 1, in vitro endotoxin experiment

[0023] 1.1 Experimental materials

[0024] All Chinese herbal medicines used were identified as genuine products; freeze-dried Escherichia coli endotoxin standard (Beijing Institute of Biological Products Inspection); Limulus reagent (TAL) (Zhanjiang Andus Biotechnology Company); Dusi Biological Co., Ltd.); endotoxin quantitative assay kit (Shanghai Yihua Biological Products Co., Ltd.); endotoxin (Sigma); polymyxin B (Sigma); transmission electron microscope (H-600 Hitachi).

[0025] 1.2 Method:

[0026] 1.2.1 preparation preparation

[0027] The anti-endotoxemia pharmaceutical composition by weight: capillary 20%, gardenia 20%, rhubarb 10%, salvia miltiorrhiza 9%, honeysuckle 20%, forsythia 9%, sunburned astragalus 9%, licorice or roasted licorice 3 % Combined deployment, except for rhubarb, decoct 3 times, 20 minutes each time, combine the decoction to 1 / 2-1 / 6 volume, add rhubarb and decoct for 40-50 minutes, and then d...

Embodiment 2

[0033] Embodiment 2, in vivo anti-endotoxin activity experiment

experiment approach I

[0034] 2.1 Experimental scheme I: Modeling with endotoxin + lead acetate (PbAC)

[0035] 2.1.1 Experimental materials

[0036] The anti-endotoxemia pharmaceutical composition by weight: capillary 20%, gardenia 20%, rhubarb 10%, salvia miltiorrhiza 9%, honeysuckle 20%, forsythia 9%, sunburned astragalus 9%, licorice or roasted licorice 3 % Combined deployment, except for rhubarb, decoct 3 times, 20 minutes each time, add rhubarb and decoct for 40-50 minutes, combine the decoction to contain 1 gram of crude drug per milliliter. Endotoxin (LPS Sigma Company), lead acetate (PbAC analytically pure), Kunming white mice, half male and half female, body weight (20±2) g, were purchased from the Experimental Animal Center of Hubei Province.

[0037] 2.1.2 Experimental methods and results

[0038] Accurately weigh the endotoxin and make a 0.4 mg / ml solution with saline. Accurately weigh PbAC and make a 4 mg / ml solution with double distilled water. 30 mice were divided into 3 groups, ...

Technical field

[0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a compound pharmaceutical preparation of an anti-endotoxemia traditional Chinese medicine composition. Background technique

[0002] Endotoxins are lipopolysaccharides that make up the cell membranes of Gram-negative bacteria. It has a double impact on the body: a small amount of endotoxin stimulation can enhance the body's specific and non-specific immunity, induce interferon, etc., while a large amount of endotoxin entering the circulatory system will cause endotoxemia, which can cause a wide range of pathological reactions. Such as multiple organ dysfunction syndrome, disseminated intravascular coagulation, multiple organ failure and shock. Septic shock and subsequent multiple organ dysfunction syndrome are the main causes of death in patients with sepsis. As a means for preventing and treating endotoxemia, antibiotics have conventionally been...

Examples

Embodiment 1

[0022] Embodiment 1, in vitro endotoxin experiment

[0023] 1.1 Experimental materials

[0024] All Chinese herbal medicines used were identified as genuine products; freeze-dried Escherichia coli endotoxin standard (Beijing Institute of Biological Products Inspection); Limulus reagent (TAL) (Zhanjiang Andus Biotechnology Company); Dusi Biological Co., Ltd.); endotoxin quantitative assay kit (Shanghai Yihua Biological Products Co., Ltd.); endotoxin (Sigma); polymyxin B (Sigma); transmission electron microscope (H-600 Hitachi).

[0025] 1.2 Method:

[0026] 1.2.1 preparation preparation

[0027] The anti-endotoxemia pharmaceutical composition by weight: capillary 20%, gardenia 20%, rhubarb 10%, salvia miltiorrhiza 9%, honeysuckle 20%, forsythia 9%, sunburned astragalus 9%, licorice or roasted licorice 3 % Combined deployment, except for rhubarb, decoct 3 times, 20 minutes each time, combine the decoction to 1 / 2-1 / 6 volume, add rhubarb and decoct for 40-50 minutes, and then d...

Embodiment 2

[0033] Embodiment 2, in vivo anti-endotoxin activity experiment

experiment approach I

[0034] 2.1 Experimental scheme I: Modeling with endotoxin + lead acetate (PbAC)

[0035] 2.1.1 Experimental materials

[0036] The anti-endotoxemia pharmaceutical composition by weight: capillary 20%, gardenia 20%, rhubarb 10%, salvia miltiorrhiza 9%, honeysuckle 20%, forsythia 9%, sunburned astragalus 9%, licorice or roasted licorice 3 % Combined deployment, except for rhubarb, decoct 3 times, 20 minutes each time, add rhubarb and decoct for 40-50 minutes, combine the decoction to contain 1 gram of crude drug per milliliter. Endotoxin (LPS Sigma Company), lead acetate (PbAC analytically pure), Kunming white mice, half male and half female, body weight (20±2) g, were purchased from the Experimental Animal Center of Hubei Province.

[0037] 2.1.2 Experimental methods and results

[0038] Accurately weigh the endotoxin and make a 0.4 mg / ml solution with saline. Accurately weigh PbAC and make a 4 mg / ml solution with double distilled water. 30 mice were divided into 3 groups, ...