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Xylose staphylocci for degrading 2-picoline and its use

A Staphylococcus xylosus, picoline technology, applied in Staphylococcus xylosus and its application fields, can solve the problems of difficult biodegradation, 2-picoline degradation, no effective methods, etc.

Active Publication Date: 2007-11-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, 2-picoline is a difficult biodegradable organic compound, and it is difficult to completely and effectively degrade 2-picoline within a certain period of time by traditional treatment methods
And for the control of soil and gas polluted by pyridines, there is no good and effective method at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the isolation and cultivation of S.xylous B1 bacterial strain

[0015] 1. Isolation of strains

[0016] Sludge samples were collected at the sewage treatment plant of Fushun Petroleum No. 3 Plant. Take 1 drop of sludge aqueous solution on the screening medium, add 0.1g of 2-picoline, spread it evenly with a spreader, put it in an incubator, and cultivate it at 30°C for 2 days; use an inoculation loop to transfer the screened strains to Connected to LB medium, placed in an incubator at 30°C for 1 day, and the obtained strain was S.xylous B1 strain.

[0017] Wherein said screening medium component is:

[0018] (NH 4 ) 2 SO 4 5g; K 2 HPO 4 1g;

[0019] K H 2 PO 4 4g; NaCl 2g;

[0020] MgSO 4 ·7H 2 O 1g; Agar 15g;

[0021] Water 1000mL; pH 7.0.

[0022] 2. Culture of strains

[0023] Use an inoculation loop to inoculate the strains screened in shake flasks with a liquid volume of 50 ml, and culture them in a shaking incu...

Embodiment 2

[0030] The cultivation of the S.xylous B1 strain was the same as in Example 1. Take 2 shake flasks, add 50mL sterile water and 1mL 2-picoline respectively. One of the shake flasks was taken as a blank control group, and the cultured B1 bacterial agent was added to the other shake flask to make its OD value 4.2. The two shaking flasks were placed on a shaker to shake the reaction, and the conditions of the shaker were: temperature 30° C.; rotation speed 150 rpm. After 10 hours, samples were taken and analyzed, and the results are listed in Table 2.

[0031] initial

Embodiment 3

[0033] The cultivation of the S.xylous B1 strain was the same as in Example 1. Take 2 shake flasks, fill with 50ml of sterile water respectively, and then add 4g of soil contaminated by 1ml of 2-picoline. Take one shake flask as a blank control group; add B1 bacterial agent to the other shake flask to make its OD value 5.0. Place 2 shake flasks on a shaker to shake the reaction. Shaking table conditions are: temperature 30°C; rotation speed 150 rpm. After 10 hours, samples were taken and analyzed, and the results are shown in Table 3.

[0034] initial

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PUM

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Abstract

The Staphylococcus xylous B1 is prepared by separating out from active sludge in sewage disposal tank of petrochemical plant, purifying and culturing. The preservative number is CGMCC No. 1485. It has excellent efficient degradable performance for 2-picoline and can be used to dispose waste water and waste gas containing 2-picoline, polluted water and soil.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Staphylococcus xylosus and its application. Background technique [0002] Heterocyclic compounds are important fine chemical raw materials. Due to their unique chemical structure, they show unique properties different from other traditional benzene-based and naphthalene-based fine chemical intermediates. They are widely used in dyes, medicines, pesticides, additives, etc. field. At present, compounds with heterocyclic structures have become hot development products and development trends in the field of fine chemicals. The production and application of heterocyclic compounds will produce a lot of "three wastes", especially the large amount of waste water. With the rapid development of domestic heterocyclic compounds, the treatment of "three wastes" containing heterocyclic compounds is increasingly necessary and urgent. 2-picoline is such a heterocyclic comp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84B09C1/10C12R1/44
CPCY02A50/20
Inventor 范峰佟明友黎元生许谦
Owner CHINA PETROLEUM & CHEM CORP
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