Method for preparing general flavone and total alkaloid of sophora flavescens simultaneously

Abstract

The invention discloses a method for simultaneously preparing total flavone extract and total alkaloid extract of Radix sophorae flavescentis. Total flavone extract mainly comprises flavanones (flavanonols) such as norkurarinone, kurarinone, and isokurarinon and other flaconoids such as trifolirhizin, xanthohumol, and kuraridin. Total alkaloid extract mainly comprises matrine n-oxide, N-oxysophocarpine, matrine, sophocarpine, sophoridine, and sophoramine. Total flavone extract and total alkaloid extract can be extracted through one or more of methods of solvent-extraction, solvent extraction,macroporous adsorbent resin, extraction by supercritical fluid, column chromatography, and liquid-liquid countercurrent distribution chromatography. Flavonoid content in total flavone extract is 5-100wt%, wherein the content of norkurarinone, xanthohumol, kuraridin, and trifolirhizin occupies 1-100wt%.Alkaloid content in total alkaloid extract is 5-100wt%, wherein the content of matrine n-oxide, N-oxysophocarpine, matrine and sophocarpine occupies 5-100wt%.

Description

technical field

[0001] The invention belongs to the technical field of medicine. Specifically, it relates to a preparation method, a quality control method for simultaneously preparing a total flavonoid extract of Sophora flavescens and a total alkaloid extract of Sophora flavescens, and its application in the fields of medicines, health products and cosmetics. Background technique

[0002] Sophora flavescens is the dried root of Sophora flavescens Ait., a plant belonging to the genus Sophora, and has the effects of clearing away heat and dampness, killing insects and diuretics. Sophora flavescens contains a large amount of flavonoids and alkaloids, which have antibacterial, insecticidal, antiviral, anti-inflammatory and other effects, and are the main material basis for the efficacy of Sophora flavescens. At present, there is no report on the simultaneous extraction, separation and preparation of the total flavonoid extract of Sophora flavescens and the total alkaloid extr...

Examples

Embodiment 2

[0067] Example 2: Preparation technology of total flavonoid extract and total alkaloid extract of Sophora flavescens

[0068] Sophora flavescens dried root 1kg, 70% ethanol 12L, reflux extraction 3 times, each extraction 1.5 hours, the solvent is recovered under reduced pressure to obtain the extract, add water to disperse (the solution concentration is 30mg / mL), centrifuge (3000rpm, 30min), get Aqueous solution and residue, add 20 times the amount of 98% ethanol to the residue for ultrasonic dissolution, centrifuge (3000rpm, 30min) to obtain the residue and ethanol lysate, recover the solvent from the ethanol lysate until there is no alcohol smell, add 12 times the amount of 0.7% HCl for ultrasonic dissolution, centrifuge (3000rpm, 30min), residue and acid water solution were obtained, the residue was neutralized by washing with water, and dried under reduced pressure to obtain the total flavonoid extract of Sophora flavescens. Combine acid water solution and aqueous solution...

Embodiment 3

[0069] Example 3: Preparation technology of total flavonoid extract and total alkaloid extract of Sophora flavescens

[0070]Sophora flavescens dried root 1kg, 70% ethanol 14L, reflux extraction 3 times, each extraction 1 hour, the solvent is recovered under reduced pressure to obtain the extract, add water to disperse (the solution concentration is 25mg / mL), centrifuge (3000rpm, 30min), get Aqueous solution and residue, add 15 times the amount of 99% ethanol to the residue for ultrasonic dissolution, centrifuge (3000rpm, 30min) to obtain the residue and ethanol lysate, recover the solvent from the ethanol lysate until there is no alcohol smell, add 9 times the amount of 0.5% HCl for ultrasonic dissolution, centrifuge (3000rpm, 30min), residue and acid water solution were obtained, the residue was neutralized by washing with water, and dried under reduced pressure to obtain the total flavonoid extract of Sophora flavescens. Combine acid water solution and aqueous solution, adj...

Embodiment 5

[0073] Example 5: Preparation of Sophora Radix total flavonoids extract tablets

[0074] Sophora flavanoid extract 100g

[0075] Starch 100g

[0076] The above components are mixed evenly, an appropriate amount of talc is added, and 1000 tablets are pressed.

technical field

[0001] The invention belongs to the technical field of medicine. Specifically, it relates to a preparation method, a quality control method for simultaneously preparing a total flavonoid extract of Sophora flavescens and a total alkaloid extract of Sophora flavescens, and its application in the fields of medicines, health products and cosmetics. Background technique

[0002] Sophora flavescens is the dried root of Sophora flavescens Ait., a plant belonging to the genus Sophora, and has the effects of clearing away heat and dampness, killing insects and diuretics. Sophora flavescens contains a large amount of flavonoids and alkaloids, which have antibacterial, insecticidal, antiviral, anti-inflammatory and other effects, and are the main material basis for the efficacy of Sophora flavescens. At present, there is no report on the simultaneous extraction, separation and preparation of the total flavonoid extract of Sophora flavescens and the total alkaloid extr...

Examples

Embodiment 2

[0067] Example 2: Preparation technology of total flavonoid extract and total alkaloid extract of Sophora flavescens

[0068] Sophora flavescens dried root 1kg, 70% ethanol 12L, reflux extraction 3 times, each extraction 1.5 hours, the solvent is recovered under reduced pressure to obtain the extract, add water to disperse (the solution concentration is 30mg / mL), centrifuge (3000rpm, 30min), get Aqueous solution and residue, add 20 times the amount of 98% ethanol to the residue for ultrasonic dissolution, centrifuge (3000rpm, 30min) to obtain the residue and ethanol lysate, recover the solvent from the ethanol lysate until there is no alcohol smell, add 12 times the amount of 0.7% HCl for ultrasonic dissolution, centrifuge (3000rpm, 30min), residue and acid water solution were obtained, the residue was neutralized by washing with water, and dried under reduced pressure to obtain the total flavonoid extract of Sophora flavescens. Combine acid water solution and aqueous solution...

Embodiment 3

[0069] Example 3: Preparation technology of total flavonoid extract and total alkaloid extract of Sophora flavescens

[0070]Sophora flavescens dried root 1kg, 70% ethanol 14L, reflux extraction 3 times, each extraction 1 hour, the solvent is recovered under reduced pressure to obtain the extract, add water to disperse (the solution concentration is 25mg / mL), centrifuge (3000rpm, 30min), get Aqueous solution and residue, add 15 times the amount of 99% ethanol to the residue for ultrasonic dissolution, centrifuge (3000rpm, 30min) to obtain the residue and ethanol lysate, recover the solvent from the ethanol lysate until there is no alcohol smell, add 9 times the amount of 0.5% HCl for ultrasonic dissolution, centrifuge (3000rpm, 30min), residue and acid water solution were obtained, the residue was neutralized by washing with water, and dried under reduced pressure to obtain the total flavonoid extract of Sophora flavescens. Combine acid water solution and aqueous solution, adj...

Embodiment 5

[0073] Example 5: Preparation of Sophora Radix total flavonoids extract tablets

[0074] Sophora flavanoid extract 100g

[0075] Starch 100g

[0076] The above components are mixed evenly, an appropriate amount of talc is added, and 1000 tablets are pressed.

Images