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Tissue culture breeding method for habenaria dentata

A technology of tissue culture and culture conditions, applied in the field of plant tissue culture, can solve the problems of lack of seedlings, difficulty in breeding of phoenix japonica, etc.

Inactive Publication Date: 2008-01-09
贵州省生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a method for tissue culture and propagation of Jade phoenix to solve the problems of difficulty in breeding and lack of seedlings of J.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Collection of seeds

[0017] Collect the capsules of the mature seeds of Jasmine japonica in the field, bring them back to the laboratory, disinfect the surface with 75% alcohol cotton balls, and store them in a refrigerator at 4°C.

[0018] 2. Aseptic sowing of seeds and induction of protocorms

[0019] (1) Disinfection treatment of explants

[0020] The uncracked capsules of the collected and preserved wild Elephantium gooseberry were first sterilized with 75% alcohol for 20-30s, then sterilized with 0.1% mercuric chloride for about 15 minutes, and then rinsed with sterile water for 5-6 times. The sterilized filter paper was blotted to dry the surface moisture for later use.

[0021] (2) Aseptic sowing and cultivation of seeds

[0022] Cut the sterilized capsule longitudinally with a sterile scalpel, spread the tiny seeds inside it evenly on the solid medium of 1 / 2MS+NAA 0.5mg / L+3% sucrose+0.2% activated carbon, pH 5.8-6.0. Place them under the conditions of l...

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Abstract

The present invention is characterized by that it utilizes wild habenaria dentate seed to make aseptic sowing, utilizes seed germination induction to form protocorm, then makes protocorm propagation and differentiation, finally makes propagation of test tube plantlet.

Description

1. Technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a tissue culture and propagation method and a culture medium for Jade phoenix flower. 2. Background technology [0002] Habenaria dentata (Habenaria dentata) is a plant of the genus Habenaria dentata in the Orchidaceae. [0003] The ecological environment for the growth of Elephantium gooseberry is relatively special, and the plant grows slowly; it mainly reproduces by underground tubers. One seedling only produces one new tuber a year, and one tuber only germinates and grows to form one plant, and the reproduction coefficient is extremely low; The flowering and seed setting rate is low, and most of them are stunted, and it is extremely difficult to germinate and form new plants under natural conditions. For a long time, folks have relied on digging wild resources for medicinal purposes, resulting in the depletion of resources. The plant tissue cultu...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 陈娅娅刘作易毛堂芬
Owner 贵州省生物技术研究所
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