Application of flax root total flavone in preparing medicament for preventing and/or treating hepatitis
A technology of total flavonoids and flax, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc.
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Embodiment 1
[0036] The preparation of embodiment 1 flax root total flavonoids
[0037] 200 kg of flax (Linum usitatissimum L.) roots were extracted three times with 85% ethanol under reflux, the extracts were combined, and the ethanol was recovered under reduced pressure to obtain a crude extract.
[0038] The crude extract was dissolved in water, then extracted three times with ethyl acetate of the same volume as water, and the ethyl acetate portion was discarded.
[0039] The aqueous layer was extracted three times with the same volume of n-butanol; the n-butanol layers were combined.
[0040] The n-butanol extract was first extracted three times with the same volume of 5% aqueous sodium bicarbonate solution, and then extracted with water until neutral.
[0041] The solvent is recovered under reduced pressure to obtain the n-butanol extract, and the flax root n-butanol extract is obtained, and a yellow precipitate is precipitated, and filtered to obtain the total flavonoids of the flax...
Embodiment 2
[0042] Embodiment 2, study on the acute toxicity of flax root total flavonoids
[0043] (1) Test animals
[0044] Kunming mice, male, weighing 18-22 grams, provided by the Institute of Experimental Animals, Chinese Academy of Medical Sciences, certificate number: SCDK (Beijing) 2004-0001
[0045] (2) Test drugs
[0046] Total flavonoids of flax root, yellow powder, stored in the dark at 2-5°C, provided by Professor Qin Hailin of our institute, prepared into 250mg / ml suspension with 0.5% CMC-Na.
[0047] (3) Test method
[0048] 10 mice were orally administered total flax root flavonoids at a concentration of 250 mg / ml, and each mouse was gavaged with a volume of 1.0 ml (ie the highest concentration and maximum gavage volume). Within 7 days after administration, the signs and death of the mice were observed.
[0049] (4) Test results
[0050] After oral administration of flax root total flavonoids in mice, the signs did not change significantly. No deaths occurred.
[00...
Embodiment 3
[0053] Example 3, the effect of flax root total flavonoids on ConA-induced acute liver injury in mice
[0054] (1) Test animals
[0055] Kunming mice, male, weighing 18-22 grams, provided by the Institute of Experimental Animals, Chinese Academy of Medical Sciences, certificate number: SCDK (Beijing) 2004-0001
[0056] (2) Test drugs
[0057] Total flavonoids of flax root, yellow powder, stored in the dark at 2-5°C, provided by Professor Qin Hailin of our institute, prepared into 250mg / ml suspension with 0.5% CMC-Na.
[0058] (3) Positive control drug
[0059] Bicyclol, white powder, provided by Peking Union Medical College Pharmaceutical Factory, batch number 20030801, suspended with 0.5% CMC-Na when used.
[0060] (4) Test method
[0061] The animals were randomly divided into 6 groups, 7 animals in each group, which were normal control group, ConA model group, high-dose total flax root flavonoids administration group, medium-dose flax root total flavonoids administratio...
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