Method for expressing exogenesis genes by using hainan rubber algae

A technology of exogenous genes and rubber, applied to other methods of inserting foreign genetic materials, using vectors to introduce foreign genetic materials, single-cell algae, etc., can solve the problems of high cost, expensive jasmonic acid, and no progress, etc., to increase production , high economic value and social development prospects

Inactive Publication Date: 2008-03-12
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, since jasmonic acid is extremely expensive, it is too expensive to directly spray rubber trees
Exploring jasmonic acid substitutes to promote milk duct differentiation is one of the ways to solve the problem, but no progress has been made so far

Method used

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Embodiment Construction

[0011] The present invention will be further described below.

[0012] The method for expressing exogenous genes by using rubber algae provided by the present invention includes the pretreatment process, genetic transformation process and post-transformation cultivation and screening process of rubber algae.

[0013] 1), the pretreatment process of rubber algae: Suspension culture of rubber algae in MS liquid medium for 3-7 days, culture temperature 25-30 ℃, light intensity 1000-3000lex, shaker speed 150-250 rpm ; The rubber algae cells were collected by centrifugation. Or use the semi-solid MS medium to culture Heveta hainanensis for 6-12 days, the culture temperature is 25-30° C., and the light intensity is 1000-3000 lex; the algae cells are scraped from the surface of the semi-solid medium. Wash the collected algae cells with sterile distilled water for 2-3 times, and then wash them twice with 10% glycerin aqueous solution. After each washing, the cells should be collected...

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Abstract

The invention discloses a method for representing alien gene, wherein, a single-cell green alga (Heveochlorella hainangensis Zhang) symbiotic with rubber trees is used to represent alien gene, the procedures include pre treating Heveochlorella hainangensis Zhang, heredity transforming, culturing and sieving after transformation. The method provides a technical means for biologically synthesizing relative gene and for synthesizing jasmine acid through representing the jasmine acid by Heveochlorella hainangensis Zhang so as to improve the latex output of rubber trees, and is of high economy value and social development prospect.

Description

technical field [0001] The invention relates to a gene expression method. In particular, it relates to a method for expressing exogenous genes by unicellular green algae symbiotic with rubber trees. Background technique [0002] Natural rubber has an important strategic position in national security and economic construction, and is a strategic material related to the national economy and people's livelihood. my country's natural rubber industry was established and developed in the early 1950s. After several generations of efforts, by the 1990s, my country's natural rubber self-sufficiency rate reached 50%. However, due to the development of the automobile industry, in recent years, my country's demand for natural rubber has increased rapidly at an average annual rate of 20%, reaching 1.72 million tons in 2003. Although in 2003, my country's rubber planting area increased to 9.5 million mu (630,000 hectares), and the total output of dry rubber increased to 560,000 tons, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/13C12N15/87C12N15/79
Inventor 张家明孙雪飘庞道标谭德冠
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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