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Method for rapid measuring allantoin content in yam using near infrared spectrum

A technology of near-infrared spectroscopy and near-infrared spectrometer, which is applied in the field of medicine and can solve the problems of cumbersome inspection process, long time-consuming, high cost, etc.

Inactive Publication Date: 2008-07-30
HENAN UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, thin-layer chromatography, high-performance liquid chromatography, and capillary electrophoresis are often used to determine the content of allantoin, among which high-performance liquid chromatography is the most commonly used, but these conventional quality evaluation methods have many limitations. The pre-treatment is complex, the inspection process is cumbersome, time-consuming, expensive, and pollutes the environment, which cannot quickly reflect the intrinsic quality of yam
Most of the existing detection methods are off-line detection, which requires corresponding pretreatment of the samples to be analyzed, and cannot provide timely feedback on quality problems. Improving production efficiency is not suitable for the needs of the modern production and development of traditional Chinese medicine

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  • Method for rapid measuring allantoin content in yam using near infrared spectrum
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  • Method for rapid measuring allantoin content in yam using near infrared spectrum

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Embodiment Construction

[0004] Specific embodiments of the present invention will be described in detail below.

[0005] The present invention is realized by the following steps:

[0006] 1. To establish a calibration model, the method is to collect a yam calibration sample set, and use a near-infrared spectrometer to collect data for each sample in the calibration set to obtain the original spectral data of yam. After spectral preprocessing and spectral region selection, the allantois in yam is obtained. Utilize HPLC to measure and correct the allantoin content of sample concentration simultaneously, adopt the PLS method to establish the correction model correspondingly with the characteristic spectrum of allantoin content in the allantoin content in HPLC with the PLS method, standby;

[0007] 2. Select the test sample set, use the near-infrared spectrometer to scan, and measure the original spectrum of the yam spectrum in the test set. After spectral preprocessing and spectral region selection, the...

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Abstract

The invention relates to a method for quickly measuring the allantoin content in yam with near infrared spectrum, and the method can effectively solve the problems of fast measure of the allantoin content in the yam and improved productivity effect. The method includes the steps that: firstly, a straightening mould is built, a yam sample is collected to be used as a sample collection, a near infrared spectrogram for straightening the sample collection is obtained through scanning, the obtained spectroscopic data are performed pretreatment of spectrum, then the analysis result of a routine analysis-high performance liquid chromatography is adopted as a reference value, a least square method in the stoichiometry is applied to build the straightening mould between a yam medicinal herb spectrum and the allantoin content inside therein, the allantoin content in the yam is obtained through the measure of the straightening mould, the input of the spectroscopic data is realized by a computer and the soft thereof, the whole process has short time, fast speed and nicety, measures on line, improves the productive effect and saves a large number of manpower and material resources, is an innovation for measuring the allantoin content in the yam, and has huge economic and social benefit.

Description

1. Technical field [0001] The invention relates to the field of medicine, in particular to a method for quickly determining the content of allantoin in yam by near-infrared spectroscopy. 2. Background technology [0002] Yam (Rhizoma Dioscoreae) is the dry rhizome of Dioscorea Opposita Thunb., which has the functions of invigorating the spleen and stomach, promoting body fluid and benefiting the lungs, and invigorating the kidney and astringent essence. Down, frequent urination, deficiency heat and thirst. Allantoin is a component with a relatively high content in yam. Experimental and clinical studies have shown that allantoin has anti-irritant, anesthetic and analgesic, anti-inflammatory and antibacterial effects. It is often used to treat chapped hands and feet, ichthyosis, and multiple keratosis. skin disease. In recent years, thin-layer chromatography, high-performance liquid chromatography, and capillary electrophoresis are often used to determine the content of alla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/74
Inventor 白雁王东陈志红龚海燕宋瑞丽
Owner HENAN UNIV OF CHINESE MEDICINE
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