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Viral capsid mosaic protein HPV16 L1-P<D>, preparation method and application thereof

An HPV16L1-PD, chimeric protein technology, applied in the field of genetic engineering, can solve problems such as no research report on the preparation method of SLE therapeutic pseudovirus vaccine, and achieve guaranteed killing specificity, lower titer, and weak immunogenicity Effect

Inactive Publication Date: 2008-12-03
ARMY MEDICAL UNIV
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  • Abstract
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Problems solved by technology

But so far, there is no research report about the SLE therapeutic pseudovirus vaccine and its preparation method.

Method used

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  • Viral capsid mosaic protein HPV16 L1-P&lt;D&gt;, preparation method and application thereof
  • Viral capsid mosaic protein HPV16 L1-P&lt;D&gt;, preparation method and application thereof
  • Viral capsid mosaic protein HPV16 L1-P&lt;D&gt;, preparation method and application thereof

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Embodiment Construction

[0042] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the preferred embodiments are only for illustrating the present invention, but not for limiting the protection scope of the present invention. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0043] (1) Viral capsid chimeric protein HPV16 L1-P D preparation of

[0044] 1. HPV16 L1-P D Gene construction

[0045] According to the principle of overlapping extension PCR (Overlap-extension PCR, OE-PCR) site-directed mutagenesis, 4 primers were designed and synthesized: the outer forward primer F is shown in SEQ ID No....

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Abstract

The invention belongs to the gene engineering field, and discloses a capsid chimeric protein HPV16 L1-PD, the preparation method thereof and the application thereof. HPV16 L1-PD has an amino acid sequence shown as SEQ ID No.2; and the encoding gene thereof has a nucleotide sequence shown as SEQ ID No.1. The preparation method comprises the steps as follows: constituting HPV16 L1-PD gene; cloning the HPV16 L1-PD gene; constituting a recombination carrier pFastBacDual HPV16 L1-PD; preparing the recombined baculovirus containing the HPV16 L1-PD gene; expressing in insect cells; purifying HPV16 L1-PD, etc. HPV16 L1-PD can be adopted as a gene or a pharmaceutical target carrier and a vaccine transfer tool, and can be applied in preparing the therapeutic virus-like vaccine of systemic lupus erythematosus.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the virus capsid chimeric protein HPV16 L1-P D And its preparation method and use. Background technique [0002] Virus-like particles (VLPs) are spherical particles that are similar in structure to viruses but do not contain viral genomes. According to the source, it can be divided into virus-derived VLPs and artificial VLPs. The former is mainly prepared through genetic engineering, while the latter is artificially chemically synthesized. The surface components of VLPs can be modified flexibly to meet different needs; under suitable conditions, VLPs can encapsulate nucleic acids, drugs or other small molecular substances as molecular targeting delivery vehicles; VLPs also have unique immunological properties, Not only can it exert an adjuvant effect by affecting antigen-presenting cells, but it can also be used as an integration platform for other adjuvants, peptide vaccine...

Claims

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Application Information

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IPC IPC(8): C07K14/025C12N15/37C12N15/866A61K48/00A61K47/42A61P37/06
Inventor 杨曌王惠明吴玉章
Owner ARMY MEDICAL UNIV
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