Method for enhancing microbe oil fermentation production efficiency

A microbial oil and production efficiency technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of improving oil production efficiency, shortening fermentation time, enhancing utilization ability and oil accumulation ability

Inactive Publication Date: 2011-04-20
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention just applies this theory to microbial fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Inoculate the seed solution of Lipomyces starkeyi AS 2.1560 into the fermentation broth, incubate with ventilation at 30°C for 12 hours, then add p-hydroxyphenylethyl alcohol to a final concentration of 100 μM, cultivate for 110 hours, and collect the bacteria by centrifugation at 6000rpm for 10 minutes. The obtained dry thalline was 17.8g / L, the fat content of the thalline was 58.7%, and the fat yield coefficient was 14.9. Compared with Comparative Example 1, the fermentation time was shortened by 34 hours, and the increase rates of dry bacteria, oil content and fat yield coefficient were 1.7%, 1.6% and 3.5%, respectively.

Embodiment 2

[0024] Inoculate the seed liquid of Lipomyces starkeyi AS 2.1560 in the fermentation broth, incubate with ventilation at 30°C for 12 hours, then add tryptophan to a final concentration of 100 μM, cultivate for 115 hours, and collect the bacteria by centrifugation at 6000 rpm for 10 minutes. The obtained dry thalline was 18.0g / L, the fat content of the thalline was 60.5%, and the fat yield coefficient was 15.6. Compared with Comparative Example 1, the fermentation time was shortened by 29 hours, and the increase rates of dry cells, oil content and fat yield coefficient were 2.9%, 4.7% and 8.3%, respectively.

Embodiment 3

[0026] Inoculate the seed liquid of Lipomyces starkeyi AS 2.1560 in the fermentation broth, incubate in ventilation at 30°C for 36 hours, then add tryptol to a final concentration of 100 μM, cultivate for 111 hours, and collect the bacteria by centrifugation at 6000 rpm for 10 minutes. The obtained dry thallus was 18.3g / L, the fat content of the thallus was 62.0%, and the fat yield coefficient was 16.3. Compared with Comparative Example 1, the fermentation time was shortened by 33 hours, and the increase rates of dry cells, oil content and fat yield coefficient were 4.6%, 7.3% and 13.2%, respectively.

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Abstract

The invention relates to the production of microorganism grease, in particular to a method for improving the fermentation production efficiency of the microorganism grease which inoculates the seed of microzyme for generating grease into a fermentation liquid and adds an alcohol compound during the period of fermentation; the concentration of the alcohol compound in the fermentation liquid is 0.01 to 5.mM; the adding time of the alcohol compound is 0 to 40 h after the fermentation liquid is inoculated; after the concentration of the total reducing sugar in the fermentation liquid is reduced to be below 1g / L, the culturing is stopped; the mycelium is concentrically collected and is extracted by an organic liquid to obtain the microorganism grease; compared with a contrast group without adding the alcohol compound, the biomass of the mycelium, the grease content and the increasing rate of the fat yield factor respectively achieve 0.6 to 5.7 percent, 0.2 to 7.4 percent and 1.4 to 13.2 percent; the fermentation time is shortened by 2.8 to 27.1 percent. The method of the invention is really effective, is simply carried out and is a novel method which shortens the fermentation period, enhances the utilizing capability of the microorganism to a substrate and improves the production efficiency of the microorganism grease.

Description

technical field [0001] The present invention relates to the production of microbial oil, specifically a method for improving the production efficiency of microbial oil fermentation. During the fermentation, alcohol compounds are added to the fermentation medium, and the ventilation culture is carried out until the total reducing sugar concentration in the fermentation liquid is reduced to 1g. Stop when it is below / L, centrifuge to collect the bacteria, and extract with organic solvents to obtain higher yields of microbial oils. The technology of the invention is simple and effective, can enhance the ability of microorganisms to convert carbohydrates into oils, can shorten the fermentation cycle, and significantly improve the production efficiency of microbial oils. Background technique [0002] Microbial oil refers to some microorganisms, including yeast, mold, algae, etc., under certain conditions, convert carbohydrates, hydrocarbons, etc. into fatty acid glycerides in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 赵宗保武双
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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